Reversible Swelling and Contraction of Isolated Spinach Chloroplasts

Autor:	Shigeru I. Honda, Tasani Hongladarom
Rok vydání:	1966
Předmět:	Contraction (grammar) Sucrose biology Physiology food and beverages Articles Plant Science biology.organism_classification Osmolar Concentration Chloroplast chemistry.chemical_compound chemistry Biochemistry Genetics medicine Spinach Tonicity Mannitol Swelling medicine.symptom medicine.drug
Zdroj:	Plant Physiology. 41:1686-1694
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.41.10.1686
Popis:	By use of a micro technique for producing extracts of spinach mesophyll cells, chloroplasts were isolated in a state wherein they displayed microscopically visible, reversible osmotic properties. Swollen spherical chloroplasts treated with hypertonic sucrose or mannitol media, but not NaCl, could be shrunken to a state resembling their disk appearance in living cells. Reversible osmotic behavior was more easily demonstrated when the chloroplasts were initially isolated from cells in a relatively low osmolar concentration in contrast to using 0.25 m sucrose or more concentrated media. Individual chloroplasts could be swollen and contracted repeatedly through as many as 4 cycles. The relationship between the capacity for osmotic behavior and chloroplast appearance in cell extracts is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::faf3472fb2d325ce5454a0c4d396883e https://doi.org/10.1104/pp.41.10.1686 Zobrazit plný text záznamu