Saliva for molecular detection of SARS‐CoV ‐2 in pre‐school and school‐age children

Autor: Agnes Delaunay‐Moisan, Tiffany Guilleminot, Michaela Semeraro, Nelly Briand, Brigitte Bader‐Meunier, Romain Berthaud, Guillaume Morelle, Pierre Quartier, Caroline Galeotti, Romain Basmaci, Gregoire Benoist, Vincent Gajdos, Mathie Lorrot, Mahmoud Rifai, Matis Crespin, Zakary M'Sakni, Faheemah Padavia, Catherine Savetier‐Leroy, Michelle Lorenzi, Caroline Maurin, Sylvie Behillil, Loic de Pontual, Narcisse Elenga, Naim Bouazza, Brigitte Moltrecht, Sylvie van der Werf, Marianne Leruez‐Ville, Isabelle Sermet‐Gaudelus
Přispěvatelé: Département Plateforme (PF I2BC), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CIC - Mère Enfant Necker Cochin Paris Centre (CIC 1419), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Immunogenetics of pediatric autoimmune diseases (Equipe Inserm U1163), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Hôpital Louis Mourier - AP-HP [Colombes], Hôpital Ambroise Paré [AP-HP], AP-HP - Hôpital Antoine Béclère [Clamart], CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Ministère de l'Éducation nationale, Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Biologie des ARN et virus influenza - RNA Biology of Influenza Virus (CNRS-UMR3569), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hôpital Jean Verdier [AP-HP], Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Unité de recherche clinique / Centre d'investigation clinique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Institut Pasteur [Paris] (IP), Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Université Paris Cité (UPCité), Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), This study was funded by a research grant from the French Ministry of Health (PHRC) and sponsored by the Assistance Publique – Hôpitaux de Paris (AP-HP) and by delegation: Délégation à la Recherche Clinique et à l'Innovation - DRCI (Clinical Research and Innovation Department)., The authors thank all the families for their participation and URC-CIC Paris Centre for the implementation, monitoring and data management of the study. The later includes Jean-Marc Treluyer and Caroline Elie, Mathilde Serpinet, Céline Hauw, Amel Bouheraoua, Victor Bruyère, Mégane Ribot, Rosa Salhi, Gael Plastow, Cindy Parent, Sonia Lounis, Insaf Berrazaga, Wafa Atmani, Amélie de Haut de Sigy, Geovanna Meneses and Fabrice Gourmelon. The authors thank Carl Mann for careful reading of the manuscript.
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Environmental Microbiology
Environmental Microbiology, 2022, Thematic Issue on Pathogen and Antimicrobial Resistance Ecology, 24 (10), pp.4725-4737. ⟨10.1111/1462-2920.16151⟩
ISSN: 1462-2912
1462-2920
DOI: 10.1111/1462-2920.16151⟩
Popis: International audience; SARS-CoV-2 diagnosis is a cornerstone for the management of coronavirus disease 2019 (COVID-19). Numerous studies have assessed saliva performance over nasopharyngeal sampling (NPS), but data in young children are still rare. We explored saliva performance for SARS-CoV-2 detection by RT-PCR according to the time interval from initial symptoms or patient serological status. We collected 509 NPS and saliva paired samples at initial diagnosis from 166 children under 12 years of age (including 57 children under 6), 106 between 12 and 17, and 237 adults. In children under 12, overall detection rate for SARS-CoV-2 was comparable in saliva and NPS, with an overall agreement of 89.8%. Saliva sensitivity was significantly lower than that of NPS (77.1% compared to 95.8%) in pre-school and school-age children but regained 96% when considering seronegative children only. This pattern was also observed to a lesser degree in adolescents but not in adults. Sensitivity of saliva was independent of symptoms, in contrary to NPS, whose sensitivity decreased significantly in asymptomatic subjects. Performance of saliva is excellent in children under 12 at early stages of infection. This reinforces saliva as a collection method for early and unbiased SARS-CoV-2 detection and a less invasive alternative for young children.
Databáze: OpenAIRE
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