Identification of New Antimicrobial Peptides that Contribute to the Bactericidal Activity of Egg White against Salmonella enterica Serovar Enteritidis at 45 °C
| Autor: | Michel Gautier, Sylvie Bonnassie, Marie-Françoise Cochet, Sophie Jan, Nadine Leconte, Françoise Nau, Valérie Briard-Bion, Florence Baron, Catherine Guérin-Dubiard, Simon C. Andrews, Julien Jardin |
|---|---|
| Přispěvatelé: | Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Rennes 1 - UFR Sciences de la vie et de l'environnement (UR1 SVE), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), School of Biological Sciences, Knight Building, University of Reading (UOR), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Université de Rennes - UFR Sciences de la vie et de l'environnement (UR SVE), Université de Rennes (UR) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Serotype chemistry.chemical_classification antimicrobial activity Chemistry Salmonella enteritidis 010401 analytical chemistry Antimicrobial peptides Peptide Salmonella Enteritidis General Chemistry Antimicrobial Metrics & More Article Recommendations egg white 01 natural sciences Salmonella enterica serovar enteritidis peptide 0104 chemical sciences Microbiology Ultrafiltration (renal) [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology 13. Climate action ultrafiltration General Agricultural and Biological Sciences 010606 plant biology & botany Egg white |
| Zdroj: | Journal of Agricultural and Food Chemistry Journal of Agricultural and Food Chemistry, American Chemical Society, 2021, ⟨10.1021/acs.jafc.0c06677⟩ Journal of Agricultural and Food Chemistry, 2021, ⟨10.1021/acs.jafc.0c06677⟩ |
| ISSN: | 0021-8561 1520-5118 |
| DOI: | 10.1021/acs.jafc.0c06677⟩ |
| Popis: | International audience; A recent work revealed that egg white (EW) at 45°C exhibits powerful bactericidal activity against S. enterica serovar Enteritidis, which is surprisingly little affected by removal of the >10 kDa EW proteins. Here, we sought to identify the major EW factors responsible for this bactericidal activity by fractionating EW using ultrafiltration and nanofiltration and by characterizing the physicochemical and antimicrobial properties of the resulting fractions. In particular, 22 peptides were identified by nano-LC/MS-MS and the bactericidal activities of representative peptides (with predicted antimicrobial activity) were further assessed. Two peptides (FVPPVQR and GDPSAWSWGAEAHS) were found to be bactericidal against S. enterica serovar Enteritidis at 45°C when provided in an EW environment. Nevertheless, these peptides contribute only part of this bactericidal activity, suggesting other, yet to be determined, antimicrobial factors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|