Analysis of enrofloxacin and its metabolite ciprofloxacin in bovine and porcine muscle by high‐performance liquid chromatography following cation exchange clean‐up
| Autor: | D. J. Tyler, J. Tarbin, G. Shearer |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Swine
Health Toxicology and Mutagenesis Metabolite Quinolones Toxicology High-performance liquid chromatography chemistry.chemical_compound Anti-Infective Agents Ciprofloxacin medicine Enrofloxacin Animals Solid phase extraction Chromatography High Pressure Liquid Antibacterial agent Residue (complex analysis) Chromatography Molecular Structure Chemistry Muscles Public Health Environmental and Occupational Health Reproducibility of Results General Chemistry Chromatography Ion Exchange Clean-up Chemistry (miscellaneous) Cattle Fluoroquinolones Food Science medicine.drug |
| Zdroj: | Food Additives and Contaminants. 9:345-350 |
| ISSN: | 0265-203X |
| DOI: | 10.1080/02652039209374080 |
| Popis: | A simple and rapid method of analysis for the trace residue determination of enrofloxacin and its metabolite ciprofloxacin has been developed. Clean-up of the samples is by cation exchange solid phase extraction (SPE) and determination made by high-performance liquid chromatography using a base-deactivated column and fluorescence detection. The method has been validated for the determination of residues in bovine and porcine muscle tissue and bacon. Recoveries at the 0.010 mg kg-1 level for enrofloaxacin and ciprofloxacin respectively were 90%, 75% in bovine muscle, 75%, 54% in porcine muscle and 81%, 63% in bacon. Determination to the 0.001 mg kg-1 level in bovine muscle and to the 0.002 mg kg-1 level in porcine muscle and bacon was also carried out. The method has been used as a quantitative screening procedure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|