Behaviors of keratinocytes and fibroblasts on films of PLA50-PEO-PLA50 triblock copolymers with various PLA segment lengths

Autor: Henri Garreau, Michel Vert, Jean-Pierre Molès, Xavier Garric
Přispěvatelé: Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Laboratoire de Dermatologie Moléculaire (EA3754), Université Montpellier 1 (UM1)
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Ethylene Oxide
Keratinocytes
Magnetic Resonance Spectroscopy
Spectrophotometry
Infrared
Polymers
Cell Culture Techniques
Tetrazolium Salts
Human skin
02 engineering and technology
01 natural sciences
MESH: Tissue Engineering
Polyethylene Glycols
Contact angle
chemistry.chemical_compound
Copolymer
Glycolic acid
Skin
MESH: Ethylene Oxide
MESH: Polystyrenes
Adhesion
MESH: Tetrazolium Salts
respiratory system
021001 nanoscience & nanotechnology
MESH: Keratinocytes
MESH: Polyesters
MESH: Polymers
0210 nano-technology
Materials science
Polyesters
Biomedical Engineering
Biophysics
MESH: Thiazoles
Bioengineering
macromolecular substances
010402 general chemistry
Biomaterials
stomatognathic system
MESH: Skin
Polymer chemistry
Humans
MTT assay
Lactic Acid
Cell adhesion
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
MESH: Cell Culture Techniques
MESH: Humans
Tissue Engineering
Ethylene oxide
MESH: Spectrophotometry
Infrared
MESH: Magnetic Resonance Spectroscopy
technology
industry
and agriculture
Fibroblasts
equipment and supplies
Culture Media
0104 chemical sciences
Thiazoles
chemistry
Chemical engineering
MESH: Polyethylene Glycols
MESH: Fibroblasts
MESH: Culture Media
Polystyrenes
MESH: Lactic Acid
Zdroj: Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine, Springer Verlag, 2008, 19 (4), pp.1645-51. ⟨10.1007/s10856-007-3232-1⟩
ISSN: 0957-4530
1573-4838
DOI: 10.1007/s10856-007-3232-1⟩
Popis: International audience; The growth of human primary keratinocytes and fibroblasts on PLA-PEO-PLA copolymer films was investigated as an intermediate stage of a strategy aimed at making implantable dermo-epidermal substitutes. Four PLA-PEO-PLA triblock copolymers with the same PEO block and different DL-lactic acid/ethylene oxide molar ratios (LA/EO) (0.8, 1.4, 1.8 and 2), were synthesized and characterized by 1H-nuclear magnetic resonance and infrared spectroscopy. The films made of these copolymers were more hydrophilic than PLA50 and than tissue culture polystyrene controls according to contact angles with water. Proliferation and adhesion of human skin cells were evaluated by MTT assay and by scanning electron microscopy. The presence of PEO in the triblock copolymers influenced cell adhesion and proliferation of fibroblasts, whereas keratinocyte adhesion and proliferation were not affected. These features emphasize the interest of PLA-PEO-PLA triblock copolymers to serve as better compounds than the racemic PLA previously investigated to make supports for human skin primary cells and scaffolds for skin engineering.
Databáze: OpenAIRE
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