Angiotensin-converting enzyme inhibitor increases angiotensin type 1A receptor gene expression in aortic smooth muscle cells of spontaneously hypertensive rats
| Autor: | Yoshiharu Kanayama, Tadanao Takeda, Naohiro Umetani, Mikio Okamura, Yoshio Konishi, Junko Iwai, Takatoshi Inoue, Nobuo Negoro, Masayo Nishimura |
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| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty Angiotensin receptor Vascular smooth muscle Molecular Sequence Data Gene Expression Blood Pressure Polymerase Chain Reaction Muscle Smooth Vascular Ribonucleases Enalapril Heart Rate Rats Inbred SHR Internal medicine Renin–angiotensin system medicine Doxazosin Animals RNA Messenger Receptor Molecular Biology Antihypertensive Agents Aorta Receptors Angiotensin Base Sequence biology Body Weight Angiotensin-converting enzyme Hydralazine Rats Endocrinology Hypertension biology.protein Molecular Medicine Oligonucleotide Probes medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1226:19-24 |
| ISSN: | 0925-4439 |
| Popis: | To examine the regulation of angiotensin receptors in vascular smooth muscle cells, we studied the effects of antihypertensive drugs on angiotensin type 1A (AT1A) receptor gene expression in aortic smooth muscle cells (ASMCs) from spontaneously hypertensive rats (SHRs) using both ribonuclease protection assay and reverse-transcription polymerase chain reaction. An increase in AT1A receptor gene expression in ASMCs of SHRs was induced by treatment with an angiotensin converting enzyme inhibitor (enalapril) for 2 weeks and 4 weeks, but not by other types of antihypertensive drugs such as alpha-blocker (doxazosin), alpha, beta-blocker (arotinolol), Ca antagonist (nicardipine) or vascular smooth muscle relaxant (hydralazine). Since all antihypertensive drugs lowered the blood pressure of the rats almost equally, our results suggest that AT1A receptor gene expression in ASMCs of SHRs may be regulated by the vascular renin-angiotensin system. |
| Databáze: | OpenAIRE |
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