TNF-α messenger ribonucleic acid (mRNA) in patients with nonalcoholic steatohepatitis
| Autor: | Reem Serhal, George Hilal, Abir Abedelrahman, Salem Khoury, Nada Alaaeddine, Joseph Sidaoui |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Clinical Biochemistry Immunology Pathogenesis Non-alcoholic Fatty Liver Disease Internal medicine medicine Immunology and Allergy Humans Receptors Tumor Necrosis Factor Type II RNA Messenger Receptor Messenger RNA biology Tumor Necrosis Factor-alpha C-reactive protein Fatty liver Middle Aged medicine.disease Blood proteins Reverse transcription polymerase chain reaction Fatty Liver Endocrinology C-Reactive Protein ROC Curve Solubility Case-Control Studies biology.protein Tumor necrosis factor alpha Female |
| Zdroj: | European cytokine network. 23(3) |
| ISSN: | 1952-4005 |
| Popis: | tumor necrosis factor (TNF)-α plays a significant role in the pathogenesis of nonalcoholic steatohepatitis (NASH). A few studies have confirmed high TNF-α plasma protein levels in patients with NASH compared to healthy volunteers. We herein aimed to revisit these findings using other molecular techniques. a cross-sectional evaluation of patients newly diagnosed with NASH. A quantitative assay for the measurement of TNF-α messenger ribonucleic acid (mRNA) was performed for NASH patients and controls using real-time reverse transcription polymerase chain reaction (RT-PCR). in 39 patients with NASH (mean age 38.6 ± 9.4 years, range 28-60 years; 79% males), the mean TNF-α mRNA level was significantly higher than that found for controls (137.6 ± 102.3 ng/mL versus 83.5 ± 43.8 ng/mL, respectively; P = 0.012). A TNF-α mRNA cut-off of 100 ng/mL predicted NASH most optimally (AUC 0.685 ± 0.066, P = 0.01; with 66.7% sensitivity and 74.1% specificity). Serum TNF-α and soluble TNF-α receptor II (sTNFRII) levels were significantly higher in patients compared to controls using ELISA. high TNF-α mRNA levels, determined by RT-PCR, characterize patients with NASH. |
| Databáze: | OpenAIRE |
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