Evidence for ATP interaction with phosphatidylcholine bilayers
| Autor: | Alvaro Garcia, Simon Pochinda, Paninnguaq Naja Elgaard-Jørgensen, Ronald J. Clarke, Himanshu Khandelia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Lipid Bilayers
adenosine triphosphate Molecular Conformation Phospholipid 02 engineering and technology Molecular Dynamics Simulation 010402 general chemistry 01 natural sciences chemistry.chemical_compound Adenosine Triphosphate Phosphatidylcholine Electrochemistry General Materials Science phosphatidylcholine Spectroscopy Ion channel electrical impedance spectoscopy Sarcolemma Chemical Physics Surfaces and Interfaces Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Cytosol Membrane molecular dynamics simulation chemistry Restricted Diffusion Phosphatidylcholines Biophysics Thermodynamics differential scanning calorimetry 0210 nano-technology Adenosine triphosphate 030403 - Characterisation of Biological Macromolecules [FoR] |
| Zdroj: | Garcia, A, Pochinda, S, Elgaard-Jørgensen, P N, Khandelia, H & Clarke, R J 2019, ' Evidence for ATP Interaction with Phosphatidylcholine Bilayers ', Langmuir, vol. 35, no. 30, pp. 9944-9953 . https://doi.org/10.1021/acs.langmuir.9b01240 |
| DOI: | 10.1021/acs.langmuir.9b01240 |
| Popis: | © 2019 American Chemical Society. ATP is a fundamental intracellular molecule and is thought to diffuse freely throughout the cytosol. Evidence obtained from nucleotide-sensing sarcolemmal ion channels and red blood cells, however, suggest that ATP is compartmentalized or buffered, especially beneath the sarcolemma, but no definitive mechanism for restricted diffusion or potential buffering system has been postulated. In this study, we provide evidence from alterations to membrane dipole potential, membrane conductance, changes in enthalpy of phospholipid phase transition, and from free energy calculations that ATP associates with phospholipid bilayers. Furthermore, all-atom molecular dynamics simulations show that ATP can form aggregates in the aqueous phase at high concentrations. ATP interaction with membranes provides a new model to understand the diffusion of ATP through the cell. Coupled with previous reports of diffusion restriction in the subsarcolemmal space, these findings support the existence of compartmentalized or buffered pools of ATP. |
| Databáze: | OpenAIRE |
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