Synthesis and characterization of Bodipy functionalized magnetic iron oxide nanoparticles for potential bioimaging applications
| Autor: | R. Beklem Bostancıoğlu, A. Tansu Koparal, Seda Demirel Topel, Önder Topel |
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| Přispěvatelé: | Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Koparal, Ayşe Tansu |
| Rok vydání: | 2015 |
| Předmět: |
Boron Compounds
Fluorophore Cell Survival Cytotoxicity Bodipy Nanoparticle Multifunctional Nanoparticles Conjugated system Bio-Imaging chemistry.chemical_compound Colloid and Surface Chemistry Cell Line Tumor Human Umbilical Vein Endothelial Cells Humans Polyethyleneimine Organic chemistry Particle Size Physical and Theoretical Chemistry Magnetite Nanoparticles Fluorescent Dyes Chemistry technology industry and agriculture Magnetic Nanoparticles Surfaces and Interfaces General Medicine Ferrosoferric Oxide Molecular Imaging Covalent bond Magnetic nanoparticles Human umbilical vein endothelial cell BODIPY Iron oxide nanoparticles Biotechnology Nuclear chemistry |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 128:245-253 |
| ISSN: | 0927-7765 |
| Popis: | WOS: 000353930000032 PubMed ID: 25707751 Multifunctional magnetic nanoparticles were synthesized for potential bio-imaging applications. Uniform PEI coated magnetic Fe3O4 (PEI-Fe3O4) nanoparticles were prepared by a modified co-precipitation method and then covalently conjugated with a fluorophore molecule, Bodipy-5 by the DCC/DMAP coupling reaction. The covalent binding of Bodipy-5 to the PEI coated magnetic Fe3O4 nanoparticles were confirmed by means of FTIR and XPS measurements. The imaging ability of the Bodipy coated magnetic nanoparticles was determined on two human cancer cells, A549 (human lung adenocarcinoma epithelial) and Ishikawa (endometrial adenocarcinoma), for the first time. Cytotoxicity of BOD-MNPs was evaluated in both cancer cells and healthy human umbilical vein endothelial cell line (HUVEC) by standard MTT (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. In vitro activities of the nanoparticles were also investigated Akdeniz University Coordination Unit of Scientific Research Projects [2012.01.0115.001] The authors acknowledge the Akdeniz University Coordination Unit of Scientific Research Projects (project no. 2012.01.0115.001) for their financial support. The authors are grateful to Prof. Engin U. Akkaya (Department of Chemistry, Bilkent University, Ankara, Turkey) for laboratory facilities. The authors thank especially Mustafa Guler (UNAM, Bilkent University, Ankara, Turkey) for technical assistance with the HR-TEM measurements. |
| Databáze: | OpenAIRE |
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