Structural analysis of haemoglobin binding by HpuA from the Neisseriaceae family
| Autor: | James A. Garnett, Yingqi Xu, Chi T. Wong, Stephen Hare, Steve Matthews, Akshari Gupta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Mutant
General Physics and Astronomy Receptors Cell Surface Kingella denitrificans Plasma protein binding medicine.disease_cause Biochemistry General Biochemistry Genetics and Molecular Biology Article Microbiology Hemoglobins Protein structure Bacterial Proteins medicine Humans Multidisciplinary biology General Chemistry biology.organism_classification Neisseriaceae Neisseria gonorrhoeae 3. Good health Protein Structure Tertiary Biological sciences Kingella Crystallization Bacteria Function (biology) Protein Binding |
| Zdroj: | Nature Communications Wong, C T, Xu, Y, Gupta, A, Garnett, J A, Matthews, S J & Hare, S A 2015, ' Structural analysis of haemoglobin binding by HpuA from the Neisseriaceae family ', Nature Communications, vol. 6, 10172 . https://doi.org/10.1038/ncomms10172 |
| ISSN: | 2041-1723 |
| Popis: | The Neisseriaceae family of bacteria causes a range of diseases including meningitis, septicaemia, gonorrhoea and endocarditis, and extracts haem from haemoglobin as an important iron source within the iron-limited environment of its human host. Herein we report crystal structures of apo- and haemoglobin-bound HpuA, an essential component of this haem import system. The interface involves long loops on the bacterial receptor that present hydrophobic side chains for packing against the surface of haemoglobin. Interestingly, our structural and biochemical analyses of Kingella denitrificans and Neisseria gonorrhoeae HpuA mutants, although validating the interactions observed in the crystal structure, show how Neisseriaceae have the fascinating ability to diversify functional sequences and yet retain the haemoglobin binding function. Our results present the first description of HpuA's role in direct binding of haemoglobin. The Neisseriaceae bacteria family extract heame from the haemoglobin of its host, the HpuA protein is part of this system. Here, the authors report crystal structures of apo- and haemoglobin-bound HpuA and analyse mutants to examine the interaction between HpuA and haemoglobin. |
| Databáze: | OpenAIRE |
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