Total Internal Reflection Tip-Enhanced Raman Spectroscopy of Cytochrome c
| Autor: | Andrew Bremner, David Talaga, Renaud A. L. Vallée, Sébastien Bonhommeau, Thierry Buffeteau, Sophie Lecomte |
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| Přispěvatelé: | Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Paul Pascal (CRPP), Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Hemeprotein Surface Properties 02 engineering and technology 010402 general chemistry Photochemistry Spectrum Analysis Raman Total internal reflection 01 natural sciences digestive system Tip-enhanced Raman spectroscopy symbols.namesake Monolayer parasitic diseases medicine Molecule General Materials Science Physical and Theoretical Chemistry chemistry.chemical_classification biology Biomolecule Cytochrome c Surface-enhanced Raman spectroscopy Cytochrome C Cytochromes c biochemical phenomena metabolism and nutrition 021001 nanoscience & nanotechnology 0104 chemical sciences [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry chemistry biology.protein symbols Ferric Finite difference time domain 0210 nano-technology Raman spectroscopy medicine.drug |
| Zdroj: | Journal of Physical Chemistry Letters Journal of Physical Chemistry Letters, American Chemical Society, 2020, 11 (10), pp.3835-3840. ⟨10.1021/acs.jpclett.0c00579⟩ |
| ISSN: | 1948-7185 |
| Popis: | International audience; Surface and tip-enhanced Raman spectroscopies in total internal reflection (TIR-SERS and TIR-TERS) are used to characterize the oxidation, spin, and ligation state of cytochrome c (Cc) molecules electrostatically bound on a hydrophilic thiol self-assembled monolayer. TIR-SERS spectra of this model hemoprotein show marker bands typical of the 6cLS ferric state of Cc. The performances of the TIR-TERS technique as a function of the incidence angle are described, showing in particular a significant electromagnetic enhancement of the Raman signal under p-polarized light excitation. TIR-TERS spectra of Cc confirm the 6cLS ferric state assignment deduced from TIR-SERS spectra, thus demonstrating the possibility of probing with nanoscale spatial resolution the 6cLS oxidized form of Cc that is potentially implicated in cell apoptotic processes. The minimal far-field contribution of the sample in TIR-TERS also offers promising perspectives for future nanoscale chemical characterizations of photosensitive biomolecules in complex biological media |
| Databáze: | OpenAIRE |
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