A Critical Role for Calponin 2 in Vascular Development
| Autor: | Jian Ping Jin, Nathan Bahary, Vikas P. Sukhatme, Guang Hu, Bernard Thisse, Leonard I. Zon, Jenna L. Galloway, Jian Tang, Ganesh Yadlapalli, Sonia Sinha, Jun-ichi Hanai, Yanfeng Lin, Bo Zhang, Christine Thisse |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Signal Transduction
MESH: Amino Acid Sequence MESH: Calcium-Binding Proteins Biochemistry MESH: Oligonucleotides Antisense Cell Movement MESH: Animals Cloning Molecular Phosphorylation Extracellular Signal-Regulated MAP Kinases MESH: Cell Movement MESH: Extracellular Signal-Regulated MAP Kinases Zebrafish 0303 health sciences Gene knockdown biology Microfilament Proteins 030302 biochemistry & molecular biology MAP Kinase Kinase Kinases musculoskeletal system Calponin 2 medicine.anatomical_structure Human umbilical vein endothelial cell MESH: Endothelium Vascular MESH: Neovascularization Physiologic Signal Transduction Molecular Sequence Data Calponin Neovascularization Physiologic MESH: Zebrafish Proteins macromolecular substances Cell Line MESH: Microfilament Proteins 03 medical and health sciences medicine Animals Humans MESH: Cloning Molecular Amino Acid Sequence MESH: Zebrafish Fibroblast Protein kinase A Molecular Biology 030304 developmental biology MESH: Molecular Sequence Data MESH: Humans MESH: Phosphorylation Calcium-Binding Proteins [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology Oligonucleotides Antisense Zebrafish Proteins biology.organism_classification Molecular biology MESH: Cell Line MESH: MAP Kinase Kinase Kinases biology.protein Endothelium Vascular Wound healing |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281 (10), pp.6664-72. ⟨10.1074/jbc.M506991200⟩ |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.m506991200 |
| Popis: | International audience; Calponin 2 (h2 calponin, CNN2) is an actin-binding protein implicated in cytoskeletal organization. We have found that the expression of calponin 2 is relatively restricted to vasculature from 16 to 30 h post-fertilization during zebrafish (Danio rerio) development. Forty-eight hours after injecting antisense morpholino oligos against calponin 2 into embryos at the 1-4-cell stage, zebrafish demonstrated various cardiovascular defects, including sluggish axial and head circulation, absence of circulation in intersegmental vessels and in the dorsal longitudinal anastomotic vessel, enlarged cerebral ventricles, and pericardial edema, in addition to an excess bending, spiraling tail and twisting of the caudal fin. Knockdown of calponin 2 in the Tg(fli1:EGFP)(y1) zebrafish line (in which a fli1 promoter drives vascular-specific enhanced green fluorescent protein expression) indicated that diminished calponin 2 expression blocked the proper migration of endothelial cells during formation of intersegmental vessels. In vitro studies showed that basic fibroblast growth factor-induced human umbilical vein endothelial cell migration was down-regulated by knockdown of calponin 2 expression using an antisense adenovirus, and overexpression of calponin 2 enhanced migration and hastened wound healing. These events were correlated with activation of mitogen-activated protein kinase; moreover, inhibition of this pathway blocked the promigratory effect of calponin 2. Collectively, these data suggest that calponin 2 plays an important role in the migration of endothelial cells both in vivo and in vitro and that its expression is critical for proper vascular development. |
| Databáze: | OpenAIRE |
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