Specific detection of topoisomerase I from the malaria causing P. falciparum parasite using isothermal rolling circle amplification
| Autor: | Eskild Petersen, Birgitta R. Knudsen, Barbara Arnò, Rikke Frøhlich, Felicie F. Andersen, Magnus Stougaard, Alessandro Desideri, Paola Fiorani, Christine J. F. Nielsen, Cinzia Tesauro, Sissel Juul |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Specific detection
plasmodium falciparum malaria type I DNA topoisomerases type I nucleic acid amplification techniques humans biosensing techniques parasitic diseases medicine Parasite hosting DNA topoisomerases Detection limit biology Settore BIO/11 Topoisomerase Plasmodium falciparum biology.organism_classification medicine.disease Virology Molecular biology Diagnosis of malaria DNA Topoisomerases Type I Rolling circle replication biology.protein Malaria |
| Zdroj: | Tesauro, C, Juul, S, Arno, B, Nielsen, C J F, Fiorani, P, Frøhlich, R F, Andersen, F F, Desideri, A, Stougaard, M, Petersen, E & Knudsen, B R 2012, ' Specific detection of topoisomerase i from the malaria causing P. falciparum parasite using isothermal Rolling Circle Amplification ', I E E E Engineering in Medicine and Biology Society. Conference Proceedings, vol. 2012, pp. 2416-2419 . https://doi.org/10.1109/EMBC.2012.6346451 EMBC |
| Popis: | We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for future point-of-care diagnosis of malaria. In the developed setup, specific detection of malaria parasites in crude blood samples is facilitated by the conversion of single Plasmodium falciparum topoisomerase I (pfTopI) mediated cleavage-ligation events, happening within nanometer dimensions, to micrometer-sized products readily detectable at the single molecule level in a fluorescence microscope. In principle, REEAD requires no special equipment and the readout is adaptable to simple colorimetric detection systems. Moreover, with regard to detection limit the presented setup is likely to outcompete standard gold immuno-based diagnostics. Hence, we believe the presented assay forms the basis for a new generation of easy-to-use diagnostic tools suitable for the malaria epidemic areas in developing countries. |
| Databáze: | OpenAIRE |
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