A sensitive and specific lateral flow assay for rapid detection of antibodies against glycoprotein B of Aujeszky's disease virus
| Autor: | Oleg S. Morenkov, Pavel P. Gladyshev, Sergey V. Dezhurov, V. V. Vrublevskaya, Andrey A. Grinevich, V. N. Afanasyev, Sagila A. Ibragimova, Y. Y. Skarga, Dmitry V. Krylsky |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system Swine viruses animal diseases Enzyme-Linked Immunosorbent Assay Gold Colloid Biology Antibodies Viral Sensitivity and Specificity Virus Epitope 03 medical and health sciences chemistry.chemical_compound Affinity chromatography Viral Envelope Proteins Virology Animals Glycoprotein B Swine Diseases Pseudorabies Antibodies Monoclonal Molecular biology Herpesvirus 1 Suid 030104 developmental biology chemistry Colloidal gold biology.protein Antibody Nitrocellulose Conjugate |
| Zdroj: | Journal of virological methods. 249 |
| ISSN: | 1879-0984 |
| Popis: | A direct double antibody lateral flow assay (DDA-gB-LFA) for the detection of antibodies against the glycoprotein B (gB) of Aujeszky's disease virus (ADV) in swine sera was developed. A native ADV gB was used for the preparation of a conjugate with colloidal gold particles and the immobilization on the strip membrane. The gB purified from ADV virions by immunoaffinity chromatography retained its native epitope structure after adsorption on the nitrocellulose membrane and the surface of colloidal gold particles. The diagnostic specificity and sensitivity of the DDA-gB-LFA were evaluated using 236 field swine sera. The diagnostic specificity and sensitivity of the DDA-gB-LFA compared to a commercially available gB-based ELISA were 98.0% and 98.6%, respectively, when determined with the use of the reader-detection mode, and 98.0% and 93.5%, respectively, when determined using visual detection. The DDA-gB-LFA provides a rapid, sensitive, and specific determination of ADV gB-directed antibodies in sera and can be used for the detection of ADV-exposed swine. |
| Databáze: | OpenAIRE |
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