Controlling T cells spreading, mechanics and activation by micropatterning
| Autor: | Martine Biarnes-Pelicot, Laurent Limozin, Pierre-Henri Puech, Ambroise Wu, Olivier Theodoly, Laura Ghesquiere-Dierickx, Anaïs Sadoun, Yannick Hamon |
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| Přispěvatelé: | Adhésion et Inflammation (LAI), Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (DKFZ), Wroclaw University of Science and Technology, Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), DUMENIL, Anita |
| Rok vydání: | 2021 |
| Předmět: |
Cell biology
T-Lymphocytes Science [SDV]Life Sciences [q-bio] T cell Immunology Adaptive immunity Biophysics Lymphocyte Activation Article Ectopic Gene Expression Membrane biophysics 03 medical and health sciences 0302 clinical medicine Immune system Cell Line Tumor Calcium flux medicine Animals Humans Lymphocytes Receptor Cell Shape 030304 developmental biology 0303 health sciences Multidisciplinary biology Chemistry Cell adhesion Substrate (chemistry) Adhesion Mechanics Molecular Imaging 3. Good health [SDV] Life Sciences [q-bio] Chemotaxis Leukocyte medicine.anatomical_structure biology.protein Leukocyte Common Antigens Medicine Calcium Antibody Biomarkers 030217 neurology & neurosurgery Micropatterning |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-86133-1⟩ Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-86133-1⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-86133-1 |
| Popis: | We designed a strategy, based on a careful examination of the activation capabilities of proteins and antibodies used as substrates for adhering T cells, coupled to protein microstamping to control at the same time the position, shape, spreading, mechanics and activation state of T cells. Once adhered on patterns, we examined the capacities of T cells to be activated with soluble anti CD3, in comparison to T cells adhered to a continuously decorated substrate with the same density of ligands. We show that, in our hand, adhering onto an anti CD45 antibody decorated surface was not affecting T cell calcium fluxes, even adhered on variable size micro-patterns. Aside, we analyzed the T cell mechanics, when spread on pattern or not, using Atomic Force Microscopy indentation. By expressing MEGF10 as a non immune adhesion receptor in T cells we measured the very same spreading area on PLL substrates and Young modulus than non modified cells, immobilized on anti CD45 antibodies, while retaining similar activation capabilities using soluble anti CD3 antibodies or through model APC contacts. We propose that our system is a way to test activation or anergy of T cells with defined adhesion and mechanical characteristics, and may allow to dissect fine details of these mechanisms since it allows to observe homogenized populations in standardized T cell activation assays. |
| Databáze: | OpenAIRE |
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