Time scale performance of rapid antigen testing for SARS-CoV-2: Evaluation of 10 rapid antigen assays
| Autor: | Salah Eldin El Meshri, Adam Elzagheid, Fawzi O. Ebrahim, Khaled Ibrahim, Mohammed Ben Elfghi, Inas M Alhudiri, Abdussamee A Efrefer, Hamza Hussein Kaal, Tarek Dalyoum, Suleiman Abusrewil, Zakarya Abusrewil |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Sensitivity and Specificity SARS‐CoV‐2 COVID-19 Serological Testing Antigen Virology Internal medicine Antigen assays Nasopharynx Medicine Humans Prospective Studies Prospective cohort study Antigens Viral False Negative Reactions Research Articles Immunoassay medicine.diagnostic_test business.industry SARS-CoV-2 COVID-19 Viral Load rapid antigen tests COVID‐19 diagnosis Infectious Diseases surveillance Female business Viral load Research Article |
| Zdroj: | Journal of Medical Virology |
| ISSN: | 1096-9071 |
| Popis: | There is a great demand for more rapid tests for SARS‐CoV‐2 detection to reduce waiting time, boost public health strategies for combating disease, decrease costs, and prevent overwhelming laboratory capacities. This study was conducted to assess the performance of 10 lateral flow device viral antigen immunoassays for the detection of SARS‐CoV‐2 in nasopharyngeal swab specimens. We analyzed 231 nasopharyngeal samples collected from October 2020 to December 2020, from suspected COVID‐19 cases and contacts of positive cases at Biotechnology Research Center laboratories, Tripoli, Libya. The performance of 10 COVID‐19 Antigen (Ag) rapid test devices for the detection of SARS‐CoV‐2 antigen was compared to a quantitative reverse transcription‐polymerase chain reaction (RT‐qPCR). In this study, 161 cases had symptoms consistent with COVID‐19. The mean duration from symptom onset was 6.6 ± 4.3 days. The median cycle threshold (C t) of positive samples was 25. Among the 108 positive samples detected by RT‐qPCR, the COVID‐19 antigen (Ag) tests detected 83 cases correctly. All rapid Ag test devices used in this study showed 100% specificity. While tests from six manufacturers had an overall sensitivity range from 75% to 100%, the remaining four tests had a sensitivity of 50%–71.43%. Sensitivity during the first 6 days of symptoms and in samples with high viral loads (C t Highlights Rapid antigen testing has high sensitivity and specificity in early disease when patients present less than 7 days of symptom onsetPatients should be encouraged to test within one week after getting COVID‐19 related symptoms and within 24 hrs. if they develop disturbed smell/taste.The use of rapid antigen testing is important for reducing burden on molecular diagnostic laboratories. |
| Databáze: | OpenAIRE |
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