Virulence of Candida Auris from Different Clinical Origins in Caenorhabditis Elegans and Galleria Mellonella Host Models
| Autor: | Estibaliz Mateo, Aitzol Perez-Rodriguez, Piet W. J. de Groot, Elena Eraso, Ainara Hernando-Ortiz, Guillermo Quindós |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Immunology Virulence macromolecular substances Infectious and parasitic diseases RC109-216 Microbiology 03 medical and health sciences medicine emerging pathogen Caenorhabditis elegans 030304 developmental biology 0303 health sciences biology 030306 microbiology Host (biology) invertebrate models Invasive candidiasis Fungal pathogen Candida auris biology.organism_classification medicine.disease Pathogenicity candidiasis Galleria mellonella virulence Infectious Diseases Parasitology |
| Zdroj: | Virulence, Vol 12, Iss 1, Pp 1063-1075 (2021) Addi. Archivo Digital para la Docencia y la Investigación instname Addi: Archivo Digital para la Docencia y la Investigación Universidad del País Vasco |
| Popis: | Candida auris is an emerging multidrug-resistant fungal pathogen responsible for nosocomial outbreaks of invasive candidiasis. Although several studies on the pathogenicity of this species have been reported, the knowledge on C. auris virulence is still limited. This study aims to analyze the pathogenicity of C. auris, using one aggregating isolate and eleven non-aggregating isolates from different clinical origins (blood, urine and oropharyngeal specimens) in two alternative host models of candidiasis: Caenorhabditis elegans and Galleria mellonella. Furthermore, possible associations between virulence, aggregation, biofilm-forming capacity, and clinical origin were assessed. The aggregating phenotype isolate was less virulent in both in vivo invertebrate infection models than non-aggregating isolates but showed higher capacity to form biofilms. Blood isolates were significantly more virulent than those isolated from urine and respiratory specimens in the G. mellonella model of candidiasis. We conclude that both models of candidiasis present pros and cons but prove useful to evaluate the virulence of C. auris in vivo. Both models also evidence the heterogeneity in virulence that this species can develop, which may be influenced by the aggregative phenotype and clinical origin. This work was supported by the Euskal Herriko Unibertsitatea [PIF 16/39]; Euskal Herriko Unibertsitatea [PIF17/167]; Eusko Jaurlaritza [GIC15/78 IT-990-16]; Ministerio de Economia y Competitividad [SAF2017-86188-P]. |
| Databáze: | OpenAIRE |
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