Effect of metal chelating agents on the storage of norepinephrine in vitro by cerebral synaptic vesicles
| Autor: | R. D. Wiehle, R. W. Colburn, John M. Davis, W. H. Riesen, K. S. Rajan |
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| Rok vydání: | 1977 |
| Předmět: |
Male
Metal ions in aqueous solution Inorganic chemistry Carboxylic Acids In Vitro Techniques Inhibitory postsynaptic potential Biochemistry Synaptic vesicle Metal Norepinephrine medicine Animals Chelation Chelating Agents Pharmacology Chemistry Brain In vitro Rats Depression Chemical visual_art visual_art.visual_art_medium Amine gas treating Synaptic Vesicles medicine.drug Nuclear chemistry |
| Zdroj: | Biochemical Pharmacology. 26:1703-1708 |
| ISSN: | 0006-2952 |
| Popis: | The inhibitory effects of fourteen different metal chelating agents on the storage in vitro of [ 3 H] dl -norepinephrine (NE) by the isolated synaptic vesicles from rat brain were investigated with a view to explore the possible importance of metal chelation phenomena. The chelators under examination belong to the classes of polyamines, aminocarboxylic and hydroxy carboxylic acids exhibiting a wide range of metal-binding strengths. Values of ic 50 , viz., the amount of the chelator which will inhibit 50 per cent of the storage of [ 3 H] dl -NE, were determined for the different compounds. The strengths of chelate formation of the “synaptosomal and vesicular metal ions”, viz. Cu 2+ , Fe 2+ , Fe 3+ , Zn 2+ , Mg 2+ and Ca 2+ , with the above compounds were compared with their inhibition in vitro of the vesicular storage of [ 3 H] dl -NE in order to determine if a correlation existed between the two. On the basis of regression analyses of the data on the stabilities of the individual metal chelates (log K ML ) and the inhibitory effects of the different chelators (log ic 50 /NE), significant correlation coefficients were obtained with the Cu 2+ and Zn 2+ amine systems, i.e. 0.98 with P 2+ gave the most satisfactory correlation coefficient, i.e. 0.91, P |
| Databáze: | OpenAIRE |
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