Endothelium-derived semaphorin 3G attenuates ischemic retinopathy by coordinating β-catenin–dependent vascular remodeling
| Autor: | Nan-Nan Lu, Ying Mei Lu, Zi Zhong Hu, Qing Huai Liu, Feng Han, Kohji Fukunaga, Song Tao Yuan, Xiang Chen, Dan Yang Chen, Jun Jie Gong, Ning He Sun, Jakob Körbelin |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Endothelium Angiogenesis Mice Transgenic Semaphorins Vascular Remodeling Neovascularization Mice 03 medical and health sciences 0302 clinical medicine Retinal Diseases Semaphorin Ischemia Animals Humans Medicine beta Catenin business.industry Retinal Vessels General Medicine Diabetic retinopathy Hypoxia (medical) medicine.disease 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research Female Endothelium Vascular medicine.symptom business Blood vessel remodeling Research Article Retinopathy |
| Zdroj: | J Clin Invest |
| ISSN: | 1558-8238 0021-9738 |
| DOI: | 10.1172/jci135296 |
| Popis: | Abnormal angiogenesis and regression of the diseased retinal vasculature are key processes associated with ischemic retinopathies, but the underlying mechanisms that regulate vascular remodeling remain poorly understood. Here, we confirmed the specific expression of semaphorin 3G (Sema3G) in retinal endothelial cells (ECs), which was required for vascular remodeling and the amelioration of ischemic retinopathy. We found that Sema3G was elevated in the vitreous fluid of patients with proliferative diabetic retinopathy (PDR) and in the neovascularization regression phase of oxygen-induced retinopathy (OIR). Endothelial-specific Sema3G knockout mice exhibited decreased vessel density and excessive matrix deposition in the retinal vasculature. Moreover, loss of Sema3G aggravated pathological angiogenesis in mice with OIR. Mechanistically, we demonstrated that HIF-2α directly regulated Sema3G transcription in ECs under hypoxia. Sema3G coordinated the functional interaction between β-catenin and VE-cadherin by increasing β-catenin stability in the endothelium through the neuropilin-2 (Nrp2)/PlexinD1 receptor. Furthermore, Sema3G supplementation enhanced healthy vascular network formation and promoted diseased vasculature regression during blood vessel remodeling. Overall, we deciphered the endothelium-derived Sema3G-dependent events involved in modulating physiological vascular remodeling and regression of pathological blood vessels for reparative vascular regeneration. Our findings shed light on the protective effect of Sema3G in ischemic retinopathies. |
| Databáze: | OpenAIRE |
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