Development and validation of an SNP-based new set of markers useful for early selection for Sharka disease in apricot (P. armeniaca)
| Autor: | Véronique Decroocq, Jean Marc Audergon, Anne Bernole, Carole Confolent, Clarisse Auvinet, Naïma Dlalah, Arnaud Remay, Guillaume Roch, Patrick Lambert |
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| Přispěvatelé: | Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CEP Innovation, BioGEVES, Institut National de la Recherche Agronomique (INRA)-Ministère en charge de l'Agriculture-Groupement National Interprofessionnel des Semences, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), ISHS, Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Bordeaux (UB) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
haplotype [SDV]Life Sciences [q-bio] Disease Computational biology KASP™ Horticulture Biology 01 natural sciences marker-assisted selection resistance PRUNUS ARMENIACA L Set (abstract data type) 03 medical and health sciences [SDV.BV]Life Sciences [q-bio]/Vegetal Biology SNP ComputingMilieux_MISCELLANEOUS sharka Selection (genetic algorithm) arbre fruitier 030304 developmental biology 2. Zero hunger virus phytopathogène 0303 health sciences Plum pox virus IDENTIFICATION Haplotype KASP (TM) food and beverages POX-VIRUS-RESISTANCE fruit Marker-assisted selection SSR [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding [SDE]Environmental Sciences 010606 plant biology & botany |
| Zdroj: | Acta Horticulturae XVII International Symposium on Apricot Breeding and Culture XVII International Symposium on Apricot Breeding and Culture, ISHS, Jul 2019, Malatya, Turkey. pp.47-52, ⟨10.17660/ActaHortic.2020.1290.9⟩ 17. International Symposium on Apricot Breeding and Culture 17. International Symposium on Apricot Breeding and Culture, Jul 2019, Malatya, Turkey. pp.1, 2019 17. International Symposium on Apricot Breeding and Culture, Jul 2019, Malatya, Turkey., pp.1, 2019 |
| ISSN: | 2406-6168 0567-7572 |
| DOI: | 10.17660/actahortic.2020.1290.9 |
| Popis: | International audience; Sharka disease, due to the Plum pox virus, is a major threat to apricot trees. Most of the accessions world-wide and particularly those cultivated in Western Europe and the Mediterranean area are susceptible to the disease. A few resistance sources have been identified to date in cultivars developed in the US from a basis of Central Asian germplasm, all of these sharing the same genomic region associated with resistance to sharka in chromosome 1. Based on this, Simple sequence repeats (SSR) markers framing the MATH genes possibly responsible for the resistance have been developed in this region and a set of 2 SSRs (PGS1.21 and PGS1.24) and an indel (ZP002) was selected for identifying and discarding susceptible individuals at the plantlet stage of the apricot breeding process. However SSR markers could be difficult to implement by small teams due to the investment in hands-on time and equipment. As a consequence we turned to single nucleotide polymorphisms (SNP) to develop an alternative set of markers that could be cheaper and more easily implemented. Taking advantage of the numerous apricot genomic-sequences available at the UGAFL, we identified resistant/susceptible SNP/indel haplotypes and developed a set of three primer triplets (SP353, SP870, and SP871) based on the competitive allele-specific PCR (KASP™) technique by LGC genomics. This set was validated using a wide range of apricot accessions from different geographic origins and genetic backgrounds, concurrently with the original set of three SSRs. The new set gave similar results as the SSR-based set, was more convenient and, as a result, is now routinely used for early selection of apricot seedlings in our breeding programs. |
| Databáze: | OpenAIRE |
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