Impact of high fat diet on long non-coding RNAs and messenger RNAs expression in the aortas of ApoE(−/−) mice
| Autor: | Huaiqing Luo, Mei-Hua Bao, Jian-Ming Li, Liang Tang, Li-hua Chen, Ju Xiang, Guang-Yi Li, Li-Ping Dong, Jie Zeng, Kui-fen Ma |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Apolipoprotein E Peroxisome proliferator-activated receptor gamma Microarray Inflammation 030204 cardiovascular system & hematology Biology Diet High-Fat Calcitriol receptor Article Mice 03 medical and health sciences Apolipoproteins E 0302 clinical medicine medicine Animals Gene Regulatory Networks RNA Messenger Transcription factor Aorta Genetics Multidisciplinary Body Weight RNA Lipids Mice Inbred C57BL 030104 developmental biology TCF3 RNA Long Noncoding medicine.symptom |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep34161 |
| Popis: | Atherosclerosis is a chronic multifactorial inflammatory disease with high prevalence worldwide, and has become the leading cause of death. The present study was designed to investigate the impact of high-fat diet on ApoE(−/−) mice exhibiting atherosclerosis by detecting the genome-wide expression profile of lncRNAs and mRNAs. A total of 354 differentially expressed lncRNAs were identified (≥2.0 folds). Simultaneously, 357 differentially expressed mRNAs from the same chip were found. The expression differences of lncRNAs and mRNAs were consistent in both qPCR and microarray detection. Annotation results of the mRNAs which correlated with lncRNAs showed that the commonly related pathways were metabolism and inflammation. Hypergeometric distribution analysis indicated that the differentially expressed lncRNAs had been mostly regulated by transcription factors (TFs) such as Myod1, Rxra, Pparg, Tcf3, etc. Additional lncRNA-target-TFs network analysis was conducted for the top 20 differentially expressed lncRNAs. The results indicated Hnf4a, Ppara, Vdr, and Runx3 as the TFs most likely to regulate the production of these lncRNAs, and might play roles in inflammatory and metabolic processes in atherosclerosis. In a nutshell, the present study identified a panel of dysregulated lncRNAs and mRNAs that may be potential biomarkers or drug targets relevant to the high-fat diet related atherogenesis. |
| Databáze: | OpenAIRE |
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