The effect of infectious dose on humoral and cellular immune responses in Chlamydophila caviae primary ocular infection

Autor: Emilija Marinkovic, Marijana Stojanovic, Nadine Schuerer, Elisabeth Stein, Hadeel Chalabi, Dejana Kosanovic, Sandra Belij-Rammerstorfer, Talin Barisani-Asenbauer, Ana Filipovic, Aleksandra Inic-Kanada, Radmila Djokic, Ivana Lukic, Ehsan Ghasemian
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Eye Diseases
Neutrophils
Lymphocyte
Eye Infections
lcsh:Medicine
Chlamydia Infection
White Blood Cells
Chlamydophila caviae
Animal Cells
Immunopathology
Medicine and Health Sciences
Medicine
Lymphocytes
lcsh:Science
Immune Response
Chlamydophila Infections
Mammals
Immunity
Cellular
Multidisciplinary
Virulence
biology
Infectious dose
Animal Models
3. Good health
Infectious Diseases
medicine.anatomical_structure
Experimental Organism Systems
Vertebrates
Cellular Types
Anatomy
Research Article
Immune Cells
Guinea Pigs
Immunology
Sexually Transmitted Diseases
Dose-Response Relationship
Immunologic
Research and Analysis Methods
Rodents
Microbiology
Guinea pig
03 medical and health sciences
Immune system
Ocular System
Immunity
Animals
Animal Models of Disease
Blood Cells
business.industry
Chlamydophila
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
Eye infection
biology.organism_classification
Immunity
Humoral
Ophthalmology
Animal Models of Infection
030104 developmental biology
Amniotes
Animal Studies
Eyes
lcsh:Q
business
Head
Zdroj: PLoS ONE, Vol 12, Iss 7, p e0180551 (2017)
PLoS ONE
PLoS One
ISSN: 1932-6203
Popis: Following infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1x10(2), 1x10(4), and 1x10(6) inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4(+) and CD8(+) cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4(+) and CD8(+) cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4(+) and CD8(+) cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1x10(4), and 1x10(6) IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.
Databáze: OpenAIRE
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