Lipid Droplets Define a Sub-Population of Breast Cancer Stem Cells
| Autor: | Benjamin J Hershey, Roberta Vazzana, Débora L Joppi, Kristina M Havas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
cancer stem cells
therapeutic resistance Population lcsh:Medicine Article Flow cytometry 03 medical and health sciences 0302 clinical medicine Breast cancer breast cancer Cancer stem cell lipid Lipid droplet Medicine education 030304 developmental biology 0303 health sciences education.field_of_study medicine.diagnostic_test business.industry lcsh:R Lipid metabolism General Medicine medicine.disease Cell culture 030220 oncology & carcinogenesis Cancer research Stem cell business metabolism |
| Zdroj: | Journal of Clinical Medicine Journal of Clinical Medicine, Vol 9, Iss 1, p 87 (2019) Journal of Clinical Medicine; Volume 9; Issue 1; Pages: 87 |
| ISSN: | 2077-0383 |
| Popis: | Tumor recurrence is now the leading cause of breast cancer-related death. These recurrences are believed to arise from residual cancer stem cells that survive initial therapeutic intervention. Therefore, a comprehensive understanding of cancer stem cell biology is needed to generate more effective therapies. Here we investigate the association between dysregulation of lipid metabolism and breast cancer stem cells. Focusing specifically on lipid droplets, we found that the lipid droplet number correlates with stemness in a panel of breast cell lines. Using a flow cytometry-based method developed for this study, we establish a means to isolate cells with augmented lipid droplet loads from total populations and show that they are enriched in cancer stem cells. Furthermore, pharmacological targeting of fatty acid metabolism reveals a metabolic addiction in a subset of cell lines. Our results highlight a key role for the lipid metabolism in the maintenance of the breast cancer stem cell pool, and as such, suggest it as a potential therapeutic target. |
| Databáze: | OpenAIRE |
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