Bacillus populi sp. nov. isolated from Populus euphratica rhizosphere soil of the Taklamakan desert
| Autor: | Yu-Jing Zhu, Cetin Sengonca, Jie-ping Wang, Haifeng Zhang, Bo Liu, Guo-Hong Liu, Xiao-Ying Wang |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine China Bacillus Peptidoglycan Diaminopimelic Acid Microbiology 03 medical and health sciences chemistry.chemical_compound Cell Wall Phylogenetics RNA Ribosomal 16S Botany Phospholipids Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Base Composition Rhizosphere biology Phylogenetic tree Fatty Acids Nucleic Acid Hybridization Vitamin K 2 Sequence Analysis DNA General Medicine Ribosomal RNA 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Populus 030104 developmental biology chemistry Diaminopimelic acid Populus euphratica |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 68:155-159 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.002476 |
| Popis: | A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-45347T, was isolated from rhizosphere soil collected from the Taklamakan desert in Xinjiang (PR China). Growth was observed at 15–35 °C (optimum 25 °C), in 0 % and 20.0 % NaCl (optimum 8.0 %) and at pH 7.5–12.0 (optimum 8.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7. The main fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences affiliated FJAT-45347T to the genus Bacillus , and it showed the highest sequence similarities to Bacillus clarkii DSM 8720T (96.1 %). The average nucleotide identity and in silico DNA–DNA hybridization values between FJAT-45347T and the most closely related species were 68.5 and 26.2 %, respectively, which were lower than the thresholds commonly used to define species (96 and 70 %, respectively), indicating that it represented a member of a different taxon. The DNA G+C content was 40.6 mol%. The phenotypic characters and taxono-genomics study revealed that FJAT-45347T represents a novel species of the genus Bacillus , for which the name Bacillus populi sp. nov. is proposed. The type strain is FJAT-45347T (=DSM 104632T=CCTCC AB 2016257T). |
| Databáze: | OpenAIRE |
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