Mapping Regions of the Cauliflower Mosaic Virus ORF III Product Required for Infectivity
| Autor: | Pierre Yot, Mario Keller, Emmanuel Jacquot, Angèle Geldreich |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Recombinant Fusion Proteins
viruses Genetic Vectors Immunoblotting Molecular Sequence Data Mutant Brassica Open Reading Frames Viral Proteins Caulimovirus Virology Amino Acid Sequence Amino Acids ORFS Infectivity Sequence Homology Amino Acid biology fungi food and beverages biology.organism_classification Molecular biology Restriction site Open reading frame Mutagenesis DNA Viral Nucleic acid Cauliflower mosaic virus |
| Zdroj: | Virology. 242(2):395-402 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1997.8995 |
| Popis: | The open reading frame (ORF) III product (PIII) of the pararetrovirus cauliflower mosaic virus (CaMV) has nucleic acid-binding properties in vitro, but its biological role is not yet determined. ORF III is closely linked to ORF II and overlaps ORF IV out of frame in the CaMV genome. A new CaMV-derived vector (Ca delta) devoid of ORF III and containing unique restriction sites between ORFs II and IV was designed. Introduction of the wild-type CaMV ORF III into Ca delta results in a clone (Ca3) infectious in turnip plants. Truncated or point-mutated versions of ORF III were then inserted into Ca delta and tested in vivo. Inoculation of the different mutants into turnip revealed that the four C-terminal amino acid residues of PIII are dispensable for infectivity as well as an internal domain (amino acids 61 to 80). Taken together the results show that PIII possesses a functional two-domain organization. Moreover, the CaMV PIII function(s) cannot be replaced either by the PIII protein of another caulimovirus, the figwort mosaic virus, or by the P2 protein of the cacao swollen shoot badnavirus, a member of the second plant pararetrovirus group. |
| Databáze: | OpenAIRE |
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