| Autor: |
Fatemeh Hatefi, Hossein Asgarian-Omran, Hadi Hossein-Nataj, Armin Akbar, Ramin Shekarriz, Ehsan Zaboli, Ghasem Janbabai, Mohsen Tehrani |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Asian Pacific Journal of Cancer Prevention. 23:2225-2231 |
| ISSN: |
2476-762X |
| DOI: |
10.31557/apjcp.2022.23.7.2225 |
| Popis: |
Blockade of immune checkpoint receptors in the treatment of cancers has been mentioned in several studies. Here, we investigated the efficacy of combined blockade of two inhibitory receptors, PD-1 and TIGIT, in restoring functional features of CD8+ T-cells in CLL.CD8+ T-cells were separated from the peripheral blood of 11 CLL patients and targeted with malignant B-cells isolated from the same patients. Cells were then stimulated with anti-CD3/CD28 and PMA/ionomycin to assess their proliferative response and cytotoxic activity using MTT and CD107a degranulation assays, respectively. Cytokine production of isolated CD8+ T-cells was also determined using ELISA.There were no significant differences in proliferation and cytotoxic activity of CD8+ T-cells co-blocked with anti-PD-1/TIGIT compared to those single blocked with anti-PD-1, anti-TIGIT, or the control antibody. There was no significant difference in cytokine production of mentioned groups, either.Collectively, combined blockade of PD-1 and TIGIT failed to restore the proliferation and function of CD8+ T-cells isolated from CLL patients. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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