Impact of added copper, alone or in combination with chlortetracycline, on growth performance and antimicrobial resistance of fecal enterococci of weaned piglets
| Autor: | Robert D. Goodband, Michael D. Tokach, Joel M. DeRouchey, Steve S Dritz, Raghavendra G. Amachawadi, K. Capps, M. B. Menegat, Michael D. Apley, Kurt Perryman, Jianfa Bai, Jason C Woodworth, Brian V. Lubbers, Tiruvoor G. Nagaraja |
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| Rok vydání: | 2019 |
| Předmět: |
Chlortetracycline
Male Tetracycline Swine Microbial Sensitivity Tests 03 medical and health sciences Feces Animal science Bacterial Proteins Drug Resistance Bacterial Genetics medicine Animals 030304 developmental biology 0303 health sciences biology 030306 microbiology Chemistry Chloramphenicol General Medicine biology.organism_classification Antimicrobial Microbiology and Microbiome Animal Feed Lincomycin Anti-Bacterial Agents Diet Enterococcus Streptomycin Animal Science and Zoology Copper Food Science medicine.drug |
| Zdroj: | J Anim Sci |
| ISSN: | 1525-3163 |
| Popis: | Studies suggest a link between added copper (Cu) and co-selection of antimicrobial resistance (AMR) in Enterococcus spp., but data are inconsistent. This study aimed to assess the impact of added Cu, alone or with a feed-grade antimicrobial, on growth performance, transferable Cu resistance gene (tcrB) prevalence, abundance of tcrB in fecal community DNA, and AMR in fecal enterococci in weaned piglets. A total of 320 barrows (DNA 200 × 400, DNA Genetics) weaned at approximately 21 d of age with 7.4 kg (7.4 ± 0.06 kg) BW were used in a 28-d study. Piglets were fed a common non-medicated diet for 7 d of acclimation. Treatments were arranged in a 2 × 2 factorial design with main effects of added Cu (0 vs. 200 mg/kg Cu from Cu sulfate) and chlortetracycline (0 vs. 440 mg/kg CTC). Growth performance and fecal samples were obtained on days 0, 14, and 28. There was no evidence (P > 0.05) for Cu and CTC interaction in growth performance. Pigs fed diets with added Cu had increased (P 0.05) from days 15 to 28 and 0 to 28. Pigs fed diets with CTC had improved (P 0.05). Prevalence of tcrB-positive enterococci was higher on day 14 than other sampling days (P = 0.002). Prevalence of tetracycline resistance gene [tet(M)]-positive enterococci was not affected by treatments or day (P > 0.05). Prevalence of macrolide resistance gene [erm(B)]-positive enterococci had a significant treatment and sampling day interaction (P = 0.021). The abundance of the tcrB gene in feces, quantified by PCR, was not affected by Cu treatment. The median Cu minimum inhibitory concentrations (MIC) of tcrB-negative and -positive isolates were 3 and 20 mM, respectively (P |
| Databáze: | OpenAIRE |
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