High-Throughput Cell Concentration Using A Piezoelectric Pump in Closed-Loop Viscoelastic Microfluidics
Autor: | Jeonghun Nam, Minji Yang, Chaeseung Lim, Sungha Park, Kyounghwa Lee, Jeeyong Kim, Hyunjung Lim, Hyunseul Jee, Hyoungsook Park, Seunghee Choo |
---|---|
Rok vydání: | 2021 |
Předmět: |
concentration
Materials science Lysis Microfluidics 02 engineering and technology Hematocrit 01 natural sciences Concentration ratio Article Viscoelasticity piezoelectric pump chemistry.chemical_compound TJ1-1570 medicine Centrifugation Mechanical engineering and machinery Electrical and Electronic Engineering high-throughput closed-loop medicine.diagnostic_test Mechanical Engineering 010401 analytical chemistry Buffer solution 021001 nanoscience & nanotechnology 0104 chemical sciences Volume (thermodynamics) chemistry Control and Systems Engineering viscoelastic fluid 0210 nano-technology Biomedical engineering |
Zdroj: | Micromachines Volume 12 Issue 6 Micromachines, Vol 12, Iss 677, p 677 (2021) |
ISSN: | 2072-666X |
Popis: | Cell concentration is a critical process in biological assays and clinical diagnostics for the pre-treatment of extremely rare disease-related cells. The conventional technique for sample preconcentration and centrifugation has the limitations of a batch process requiring expensive and large equipment. Therefore, a high-throughput continuous cell concentration technique needs to be developed. However, in single-pass operation, the required concentration ratio is hard to achieve. In this study, we propose a closed-loop continuous cell concentration system using a viscoelastic non-Newtonian fluid. For miniaturized and integrated systems, two piezoelectric pumps were adopted. The pumping capability generated by a piezoelectric pump in a microfluidic channel was evaluated depending on the applied voltage, frequency, sample viscosity, and channel length. The concentration performance of the device was evaluated using 13 μm particles and white blood cells (WBCs) with different channel lengths and voltages. In the closed-loop system, the focused cells collected at the center outlet were sent back to the inlet, while the buffer solution was removed to the side outlets. Finally, to expand the clinical applicability of our closed-loop system, WBCs in lysed blood samples with 70% hematocrit and prostate cancer cells in urine samples were used. Using the closed-loop system, WBCs were concentrated by ~63.4 ± 0.8-fold within 20 min to a final volume of 160 μL using 10 mL of lysed blood sample with 70% hematocrit (~3 cP). In addition, prostate cancer cells in 10 mL urine samples were concentrated by ~64.1-fold within ~11 min due to low viscosity (~1 cP). |
Databáze: | OpenAIRE |
Externí odkaz: |