Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal PlantCimicifuga foetida
| Autor: | Qing-Zhong Wang, De-Zhu Li, Chun-Ying Xue |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cimicifuga
DNA Plant Sequence analysis Molecular Sequence Data Pharmaceutical Science Biology DNA Ribosomal Polymerase Chain Reaction Fluorescence Melting curve analysis Analytical Chemistry law.invention Intergenic region law Drug Discovery Cimicifuga foetida Medicine Chinese Traditional Internal transcribed spacer Medicinal plants Polymerase chain reaction Pharmacology Base Sequence Traditional medicine Nucleotides Organic Chemistry Sequence Analysis DNA Ribosomal RNA Complementary and alternative medicine Molecular Medicine DNA Intergenic Drugs Chinese Herbal |
| Zdroj: | Planta Medica. 75:873-875 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-0029-1185403 |
| Popis: | DNA sequence analysis of rDNA internal transcribed spacer (ITS) and fluorescence melting curve analysis of LightCycler real-time polymerase chain reaction products were exploited for their applications in the authentication of the traditional Chinese medicinal plant Cimicifuga foetida from four substitutes: C. heracleifolia, C. dahurica, C. acerina, and C. simplex. According to the melting temperature - which is a function of the GC/AT ratio, length, and nucleotide sequences of the amplified product - C. foetida was differentiated from C. heracleifolia, C. dahurica, C. acerina, and C. simplex. Melting curve analysis offers a rapid and reliable method for the authentication of the traditional Chinese medicinal plant C. foetida. |
| Databáze: | OpenAIRE |
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