Genotypes and population genetics of cryptococcus neoformans and cryptococcus gattii species complexes in Europe and the mediterranean area

Autor: Sevim Akcaglar, Wieland Meyer, Beatriz Alonso, Ana C. F. Venca, Luigi Vezzulli, Serdar Susever, Cristina Macci, Macit Ilkit, Mohamed S. Ellabib, Emilija Mlinarić-Missoni, Bart Theelen, Ana Sampaio, Nilgun Cerikcioglu, Laura Trovato, Ilka McCormick-Smith, Itzhack Polacheck, Marie Desnos-Ollivier, Giuseppe Criseo, Kennio Ferreira-Paim, Luciana Trilles, Vincenzo Pasquale, Maria Teresa Montagna, Anna Marchese, Aristea Velegraki, Volker Rickerts, Shawn R. Lockhart, Stéphane Ranque, Ludovico Dipineto, Antonella De Donno, João Inácio, Ferry Hagen, Simona Nardoni, Massimo Cogliati, María Francisca Colom, Çağrı Ergin, Sébastien Bertout, Teun Boekhout
Přispěvatelé: Università degli Studi di Milano = University of Milan (UNIMI), Institut Pasteur [Paris] (IP), Robert Koch Institute [Berlin] (RKI), The University of Sydney, The Westmead Institute for Medical Research, University of Amsterdam [Amsterdam] (UvA), Westerdijk Fungal Biodiversity Institute [Utrecht] (WI), Royal Netherlands Academy of Arts and Sciences (KNAW), University of Brighton, Universidad Miguel Hernández [Elche] (UMH), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP), Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA), Università del Salento [Lecce], Cyprus Near East University, Pamukkale University, National and Kapodistrian University of Athens (NKUA), University of Tripoli - University Al Fateh, University of Pisa - Università di Pisa, Research Institute on Terrestrial Ecosystems [CNR, Italy] (IRET), Consiglio Nazionale delle Ricerche (CNR), Università degli studi di Catania = University of Catania (Unict), University of Naples Federico II = Università degli studi di Napoli Federico II, Uludağ Üniversitesi = Uludag University, Croatian National Institute of Public Health, Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques er émergentes (TransVIHMI), Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université de Montpellier (UM), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Universidade de Trás-os-Montes e Alto Douro (UTAD), Universidade do Trás-os-Montes e Alto Douro, University of Messina, Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Marmara University [Kadıköy - İstanbul], Università degli studi di Genova = University of Genoa (UniGe), Cukurova University, Università degli Studi di Napoli 'Parthenope' = University of Naples (PARTHENOPE), Hadassah Hebrew University Medical Center [Jerusalem], Centers for Disease Control and Prevention [Atlanta] (CDC), Centers for Disease Control and Prevention, W Meyer and S Lockhart were supported by an NH&MRC grant # APP1031943., AC Sampaio was supported by Fundação para a Ciência e Tecnologia and FEDER/COMPETE/POCI provided to CITAB under the projects POCI-01-0145-FEDER-006958 and UID/AGR/04033/2019., A Velegraki was supported by SARG K.A.70/4/5905 and 70/3/6915, National and Kapodistrian University of Athens and the Bodosakis Foundation, Athens, Greece., We thank Dr. Kwon Chung and Prof. Dromer for contribution in the critical reading of the manuscript., COMBE, Isabelle, Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes (TransVIHMI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Evolutionary and Population Biology (IBED, FNWI), Università degli Studi di Milano [Milano] (UNIMI), Institut Pasteur [Paris], Westerdijk Fungal Biodiversity Insitute [Utrecht] (WI), Università degli Studi di Bari Aldo Moro, Università degli studi di Catania [Catania], University of Napoli Federico II, Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Universita degli studi di Genova, Universita degli studi di Napoli 'Parthenope' [Napoli], Cogliati, Massimo, Desnos-Ollivier, Marie, McCormick-Smith, Ilka, Rickerts, Volker, Ferreira-Paim, Kennio, Meyer, Wieland, Boekhout, Teun, Hagen, Ferry, Theelen, Bart, Inácio, Joäo, Alonso, Beatriz, Colom, Maria F., Trilles, Luciana, Teresa Montagna, Maria, De Donno, Antonella, Susever, Serdar, Ergin, Cagri, Velegraki, Aristea, Ellabib, Mohamed S., Nardoni, Simona, Macci, Cristina, Trovato, Laura, Dipineto, Ludovico, Akcaglar, Sevim, Mlinaric-Missoni, Emilija, Bertout, Sebastien, Vencá, Ana C. F., Sampaio, Ana C., Criseoa, Giuseppe, Ranquea, Stéphane, Çerikçioğlua, Nilgün, Marchesea, Anna, Vezzullia, Luigi, Ilkita, Macit, Pasqualea, Vincenzo, Polachecka, Itzhack, Lockharta, Shawn R., Westerdijk Fungal Biodiversity Institute - Yeast Research, Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Medical Mycology, Çukurova Üniversitesi, DE DONNO, Maria Antonella, Criseo, Giuseppe, Çerikçioğlu, Nilgün, Marchese, Anna, Vezzulli, Luigi, Ilkit, Macit, Pasquale, Vincenzo, Polacheck, Itzhack, Lockhart, Shawn R., Bursa Uludağ Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri/Tıbbi Mikrobiyoloji Anabilim Dalı., Akçağlar, Sevim
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Southern Europe
Epidemiology
Population genetics
genetic analysis
Population structure
Fungal gene
fungus mutation
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
genetics
Mycological Typing Techniques
Phylogeny
Cryptococcus neoformans
[SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases
genetic recombination
0303 health sciences
education.field_of_study
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
Mediterranean Region
allele
Gene linkage disequilibrium
priority journal
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Infection
Human
MLST
Species complex
Genotype
Microbiology
Article
03 medical and health sciences
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Genetics
Humans
human
education
Genetic recombination
Animal
030306 microbiology
Fungus mutation
Cryptococcus neoformans
Cryptococcus gattii
MLST
Europe
Cryptococcus gattii
Cryptococcus neoformans
Cryptococcus gattii
MLST
Population genetics
Europe
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
gene linkage disequilibrium
Genetics
Population
microbiological examination
Multilocus Sequence Typing
Europe
Cryptococcus Gattii
Flucytosine
Cryptococcus Neoformans
multilocus sequence typing
phylogeny
Genetic analysis
genetic variability
Environmental Microbiology
animal
fungal gene
Priority journal
Allele
2. Zero hunger
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
biology
Genetics & heredity
[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Microbiological examination
[SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Population
Mycology
Animals
Cryptococcus gattii/*genetics
Cryptococcus neoformans/*genetics
Cryptococcus neoformans var. neoformans
Niche
Cryptococcus neoformans var. grubii
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
PLB1 gene
030304 developmental biology
nonhuman
Hiv
population structure
LAC1 gene
Nonhuman
biology.organism_classification
Var. grub II
Clonal variation
clonal variation
Multilocus sequence typing
Genetic variability
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Zdroj: Fungal Genetics and Biology
Fungal Genetics and Biology, 2019, 129, pp.16-29. ⟨10.1016/j.fgb.2019.04.001⟩
Fungal Genetics and Biology, 129, 16-29. Academic Press Inc.
Fungal genetics and biology
129 (2019): 16–29. doi:10.1016/j.fgb.2019.04.001
info:cnr-pdr/source/autori:Cogliati, Massimo; Desnos-Ollivier, Marie; McCormick-Smith, Ilka; Rickerts, Volker; Ferreira-Paim, Kennio; Meyer, Wieland; Boekhout, Teun; Hagen, Ferry; Theelen, Bart; Inacio, Joao; Alonso, Beatriz; Colom, Maria F.; Trilles, Luciana; Montagna, Maria Teresa; De Donno, Antonella; Susever, Serdar; Ergin, Cagri; Velegraki, Aristea; Ellabib, Mohamed S.; Nardoni, Simona; Macci, Cristina; Trovato, Laura; Dipineto, Ludovico; Akcaglar, Sevim; Mlinaric-Missoni, Emilija; Bertout, Sebastien; Venca, Ana C. F.; Sampaio, Ana C.; Criseoa, Giuseppe; Ranque, Stephane; Cerikcioglu, Nilgun; Marchese, Anna; Vezzulli, Luigi; Ilkit, Macit; Pasquale, Vincenzo; Polacheck, Itzhack; Lockhart, Shawn R./titolo:Genotypes and population genetics of cryptococcus neoformans and cryptococcus gattii species complexes in Europe and the mediterranean area/doi:10.1016%2Fj.fgb.2019.04.001/rivista:Fungal genetics and biology (Print)/anno:2019/pagina_da:16/pagina_a:29/intervallo_pagine:16–29/volume:129
Fungal Genetics and Biology, Elsevier, 2019, 129, pp.16-29. ⟨10.1016/j.fgb.2019.04.001⟩
FUNGAL GENET BIOL
r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
instname
Fungal Genetics and Biology, 129, 16-29. Academic Press
ISSN: 3095-3839
1087-1845
1096-0937
DOI: 10.1016/j.fgb.2019.04.001⟩
Popis: PubMedID: 30953839 A total of 476 European isolates (310 Cryptococcus neoformans var. grubii, 150 C. neoformans var. neoformans, and 16 C. gattii species complex) from both clinical and environmental sources were analyzed by multi-locus sequence typing. Phylogenetic and population genetic analyses were performed. Sequence analysis identified 74 sequence types among C. neoformans var. neoformans (VNIV), 65 among C. neoformans var. grubii (56 VNI, 8 VNII, 1 VNB), and 5 among the C. gattii species complex (4 VGI and 1 VGIV) isolates. ST23 was the most frequent genotype (22%) among VNI isolates which were mostly grouped in a large clonal cluster including 50% of isolates. Among VNIV isolates, a predominant genotype was not identified. A high percentage of autochthonous STs were identified in both VNI (71%) and VNIV (96%) group of isolates. The 16 European C. gattii species complex isolates analyzed in the present study originated all from the environment and all belonged to a large cluster endemic in the Mediterranean area. Population genetic analysis confirmed that VNI group of isolates were characterized by low variability and clonal expansion while VNIV by a higher variability and a number of recombination events. However, when VNI and VNIV environmental isolates were compared, they showed a similar population structure with a high percentage of shared mutations and the absence of fixed mutations. Also linkage disequilibrium analysis reveals differences between clinical and environmental isolates showing a key role of PLB1 allele combinations in host infection as well as the key role of LAC1 allele combinations for survival of the fungus in the environment. The present study shows that genetic comparison of clinical and environmental isolates represents a first step to understand the genetic characteristics that cause the shift of some genotypes from a saprophytic to a parasitic life style. © 2019 Elsevier Inc. Fundação para a Ciência e a Tecnologia National and Kapodistrian University of Athens APP1031943 70/3/6915, 70/4/5905 POCI-01-0145-FEDER-006958, UID/AGR/04033/2019 We thank Dr. Kwon Chung and Prof. Dromer for contribution in the critical reading of the manuscript. The findings and conclusions in this manuscript are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention. W Meyer and S Lockhart were supported by an NH&MRC grant # APP1031943 . AC Sampaio was supported by Fundação para a Ciência e Tecnologia and FEDER/COMPETE/POCI provided to CITAB under the projects POCI-01-0145-FEDER-006958 and UID/AGR/04033/2019. A Velegraki was supported by SARG K.A.70/4/5905 and 70/3/6915, National and Kapodistrian University of Athens and the Bodosakis Foundation , Athens, Greece.
Databáze: OpenAIRE
Externí odkaz: