Knockdown of the Chromatin Remodeling Gene Brahma by RNA Interference Reduces Reproductive Fitness and Lifespan in Common Bed Bug (Hemiptera: Cimicidae)
| Autor: | Sanjay Basnet, Shripat T. Kamble |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Bedbugs animal structures Population Longevity Biology 01 natural sciences 03 medical and health sciences Bed bug RNA interference parasitic diseases Cimicidae Animals education RNA Double-Stranded Gene knockdown education.field_of_study General Veterinary Reproduction fungi biology.organism_classification Chromatin Assembly and Disassembly Hemiptera Cell biology 010602 entomology RNA silencing 030104 developmental biology Infectious Diseases Insect Science Insect Proteins Parasitology RNA Interference Genetic Fitness Cimex lectularius |
| Zdroj: | Journal of medical entomology. 55(3) |
| ISSN: | 1938-2928 |
| Popis: | The common bed bug, Cimex lectularius L. (Hemiptera: Cimicidae) is a nuisance household pest causing significant medical and economic impacts. RNA interference (RNAi) of genes that are involved in vital physiological processes can serve as potential RNAi targets for insect control. Brahma is an ATPase subunit of a chromatin-remodeling complex involved in transcription of several genes for cellular processes, most importantly the homeotic genes. In this study, we used a microinjection technique to deliver double stranded RNA into female bed bugs. Delivery of 0.05 and 0.5 µg/insect of brahma dsRNA directly into hemocele resulted substantial reduction in oviposition. Eggs laid by bed bugs receiving both doses of brahma dsRNA exhibited significantly lower hatching percentage as compared to controls. In addition, brahma RNAi in female bed bugs caused significant mortality. Our results disclosed the potential of brahma RNAi to suppress bed bug population through injection of specific dsRNA, suggesting a critical function of this gene in bed bugs' reproduction and survival. Based on our data, brahma can be a promising RNAi target for suppression of bed bug population. |
| Databáze: | OpenAIRE |
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