Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism
| Autor: | Seung-Ryoung Jung, Kenneth P. Bube, Ian R. Sweet, James B. Hurley, Varun Kamat, Brian M. Robbings, John Kelly |
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| Rok vydání: | 2021 |
| Předmět: |
Male
insulin secretion Mouse QH301-705.5 Science Cell hydrogen sulfide chemistry.chemical_element Oxygen Retina General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley Islets of Langerhans Mice medicine Animals Biology (General) Gasotransmitters calcium General Immunology and Microbiology hypoxia Chemistry General Neuroscience Pancreatic islets Cell Biology General Medicine Metabolism Hypoxia (medical) Electron transport chain Tools and Resources Rats Mice Inbred C57BL medicine.anatomical_structure Cell metabolism Liver Reperfusion Biophysics Rat Medicine Gases medicine.symptom oxygen fluidics |
| Zdroj: | eLife, Vol 10 (2021) eLife |
| ISSN: | 2050-084X |
| Popis: | Oxygen (O2) and other dissolved gases such as the gasotransmitters H2S, CO and NO affect cell metabolism and function. To evaluate effects of dissolved gases on processes in tissue, we developed a fluidics system that controls dissolved gases while simultaneously measuring parameters of electron transport, metabolism and secretory function. We use pancreatic islets, retina and liver to highlight its ability to assess effects of O2 and H2S. Protocols aimed at emulating hypoxia-reperfusion conditions resolved a previously unrecognized transient spike in O2 consumption rate (OCR) following replenishment of O2, and tissue-specific recovery of OCR following hypoxia. The system revealed both inhibitory and stimulatory effects of H2S on insulin secretion rate from isolated islets. The unique ability of this new system to quantify metabolic state and cell function in response to precise changes in dissolved gases provides a powerful platform for cell physiologists to study a wide range of disease states. |
| Databáze: | OpenAIRE |
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