Compound K induces expression of hyaluronan synthase 2 gene in transformed human keratinocytes and increases hyaluronan in hairless mouse skin
| Autor: | Yong Sung Lee, Byung Young Kang, Si Yong Cho, Dae Suk Sung, Hui Kyung Chang, Duk Hee Kim, Sujong Kim, Myung Hun Yeom, Young Chul Sim |
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| Rok vydání: | 2004 |
| Předmět: |
Keratinocytes
Ginsenosides Biophysics Gene Expression Human skin Hyaluronan Synthase 2 Biochemistry Cell Line Ginseng Mice Animals Humans Glucuronosyltransferase Hyaluronic Acid Molecular Biology Cell Line Transformed Skin Mice Hairless integumentary system Epidermis (botany) Chemistry Papillary dermis Gene Expression Profiling Cell Biology Molecular biology Hairless Up-Regulation HaCaT Cell culture Epidermis Hyaluronan Synthases |
| Zdroj: | Biochemical and biophysical research communications. 316(2) |
| ISSN: | 0006-291X |
| Popis: | Ginsenosides, the major active ingredients of ginseng, have a variety of biomedical efficacies such as anti-aging, anti-oxidation, and anti-inflammatory activities. To understand the effects of compound K (20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol), one of the major metabolites of ginsenosides, on the skin, we assessed the expression levels of about 100 transcripts in compound K-treated HaCaT cells using cDNA microarray analysis. One gene up-regulated by compound K was hyaluronan synthase2 (HAS2). Semi-quantitative RT-PCR showed that compound K increased HAS2 mRNA in time- and dose-dependent manners. ELISA and immunocytochemistry using hyaluronan (HA)-binding protein showed that compound K effectively increased HA production in HaCaT cells. Finally, treatment of compound K on hairless mouse skin increased the amount of HA in the epidermis and papillary dermis. Our study suggests that topical application of compound K might prevent or improve the deteriorations, such as xerosis and wrinkles, partly ascribed to the age-dependent decrease of the HA content in human skin. |
| Databáze: | OpenAIRE |
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