Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1
| Autor: | Joaquim Coutinho-Netto, Bruna Gabriela dos Santos Kotake, Edilson Ervolino, Miliane Gonçalves Gonzaga, João Paulo Mardegan Issa, Fellipe Augusto Tocchini de Figueiredo |
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| Přispěvatelé: | Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A medicine.medical_specialty Pathology Materials science Bone Regeneration Latex Biomedical Engineering Bioengineering Calvaria Stereology Bone healing Transplantation Autologous Biomaterials Extracellular matrix 03 medical and health sciences 0302 clinical medicine Osteogenesis bone grafts medicine Cell Adhesion Animals Transplantation Homologous Zymography Rats Wistar Bone regeneration Bone Transplantation Skull 030206 dentistry zymography Immunohistochemistry Surgery Extracellular Matrix Rats Transplantation Hevea brasiliensis 030104 developmental biology medicine.anatomical_structure Matrix Metalloproteinase 9 Peroxidases immunohistochemistry stereology Heterografts Hevea Matrix Metalloproteinase 2 |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-11-29T07:11:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Bone grafts are used in the medical-surgical field for anatomical and functional reconstruction of lost bone areas, aiding the bone repair process by osteogenesis, osteinduction and osteoconduction. New materials such as F1 (fraction 1) protein extracted from the rubber tree Hevea brasiliensis have been investigated and currently present important properties for tissue repair, and are associated with neoangiogenesis, promoting cell adhesion and extracellular matrix formation. The main objective of this study was to investigate the association of F1 protein to different bone grafts in the repair of critical bone defects in the calvaria of Wistar rats. A total of 112 Wistar rats were divided as follows: autograft (AuG), allograft (AlG), xenograft (XeG), autograft/F1 (AuG-F1), allograft/F1 (AlG-F1), xenograft/F1 (XeG-F1), F1(F1), control (CTL), with a waiting period of 4 and 6 weeks (w). The stereological AuG, AlG, AuG-F1 and AlG-F1 results had greater bone neoformation (p < 0.05). For immunohistochemistry, the angiogenic and osteogenic factors were higher for AuG-F1 and AlG-F1. TRAP positive cells were higher in XeG-F1 and AlG (37 +/- 9.53, 13.3 +/- 4.16) (4 w) and XeG, AlG-F1 and XeG-F1 (20.33 +/- 7.37; 15.25 +/- 6.02, 19.33 +/- 3.21) (6 w). For zymography, F1 showed increased gelatinolytic activity of MMP-2 and -9. It was concluded that the bone graft associated or not with F1 increases the angiogenic and osteogenic, biochemical and stereological factors. Univ Sao Paulo, Sch Med Ribeirao Preto, Ribeirao Preto, SP, Brazil Sao Paulo State Univ Julio Mesquita Filho, Aracatuba, SP, Brazil Univ Sao Paulo, Sch Dent Ribeirao Preto, Ribeirao Preto, SP, Brazil Sao Paulo State Univ Julio Mesquita Filho, Aracatuba, SP, Brazil FAPESP: 2012/03310-8 FAPESP: 2012/225321 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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