Murine colitis reveals a disease-associated bacteriophage community
| Autor: | David Paez-Espino, Lora V. Hooper, Breck A. Duerkop, Brian Bushnell, Wenhan Zhu, Nikos C. Kyrpides, Brian Hassell, Sebastian E. Winter, Manuel Kleiner |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine viruses Crohn's Disease Disease Inbred C57BL Applied Microbiology and Biotechnology Inflammatory bowel disease Genome Bacteriophage Mice 2.1 Biological and endogenous factors Bacteriophages Viral Aetiology Intestinal Mucosa Cells Cultured Mice Knockout education.field_of_study Cultured biology Colitis 3. Good health Medical Microbiology Microbiology (medical) Cells Knockout Immunology Population Genome Viral Autoimmune Disease Microbiology 03 medical and health sciences Oral and Gastrointestinal Genetics medicine Animals Humans Microbiome education Animal Inflammatory Bowel Disease Cell Biology medicine.disease biology.organism_classification Gastrointestinal Microbiome Mice Inbred C57BL Disease Models Animal 030104 developmental biology Disease Models Dysbiosis Digestive Diseases |
| Zdroj: | Nature microbiology, vol 3, iss 9 Duerkop, BA; Kleiner, M; Paez-Espino, D; Zhu, W; Bushnell, B; Hassell, B; et al.(2018). Murine colitis reveals a disease-associated bacteriophage community. Nature Microbiology, 3(9), 1023-1031. doi: 10.1038/s41564-018-0210-y. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/9n4550hk |
| ISSN: | 2058-5276 |
| DOI: | 10.1038/s41564-018-0210-y |
| Popis: | © 2018, The Author(s). The dysregulation of intestinal microbial communities is associated with inflammatory bowel diseases (IBD). Studies aimed at understanding the contribution of the microbiota to inflammatory diseases have primarily focused on bacteria, yet the intestine harbours a viral component dominated by prokaryotic viruses known as bacteriophages (phages). Phage numbers are elevated at the intestinal mucosal surface and phages increase in abundance during IBD, suggesting that phages play an unidentified role in IBD. We used a sequence-independent approach for the selection of viral contigs and then applied quantitative metagenomics to study intestinal phages in a mouse model of colitis. We discovered that during colitis the intestinal phage population is altered and transitions from an ordered state to a stochastic dysbiosis. We identified phages specific to pathobiotic hosts associated with intestinal disease, whose abundances are altered during colitis. Additionally, phage populations in healthy and diseased mice overlapped with phages from healthy humans and humans with IBD. Our findings indicate that intestinal phage communities are altered during inflammatory disease, establishing a platform for investigating phage involvement in IBD. |
| Databáze: | OpenAIRE |
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