Ageing changes the cellular basis of the 'fight-or-flight' response in human adrenal chromaffin cells
| Autor: | Clive Palfrey, Cristina R. Artalejo, Abdeladim Elhamdani |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Aging medicine.medical_specialty Patch-Clamp Techniques Adolescent Chromaffin Cells Action Potentials chemistry.chemical_element Calcium Internal medicine Adrenal Glands medicine Humans Secretion Cells Cultured Voltage-dependent calcium channel General Neuroscience Middle Aged Aggression Electrophysiology Kinetics medicine.anatomical_structure Endocrinology chemistry Ageing Chromaffin cell Catecholamine Female Calcium Channels Neurology (clinical) Geriatrics and Gerontology Adrenal medulla Developmental Biology medicine.drug Endocrine gland |
| Zdroj: | Neurobiology of Aging. 23:287-293 |
| ISSN: | 0197-4580 |
| DOI: | 10.1016/s0197-4580(01)00275-5 |
| Popis: | Stress-induced increases in plasma epinephrine in man have been reported to decrease with age. To investigate the possible cellular basis for this decline we determined the characteristics of calcium currents and their relationship to catecholamine secretion in isolated human adrenal chromaffin (AC) cells. Cells derived from young individuals displayed prominent prepulse facilitation of L-type Ca channels but this property was absent in cells from older subjects. Robust quantal secretion in young cells as determined by amperometry was strongly coupled to the activation of these channels with an average delay of only approximately 3 msec. N- and P-type Ca channels also contributed to secretion but were more weakly coupled to catecholamine release sites. Cells from older subjects secreted much less efficiently and showed only weak coupling between Ca channels and secretion. These studies suggest that the magnitude and timing of adrenal secretion changes with age and that the facilitation Ca channel is key to rapid activation of the fight-or-flight response in young individuals. |
| Databáze: | OpenAIRE |
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