Subtyping analysis reveals new variants and accelerated evolution of Clostridioides difficile toxin B
| Autor: | Liuqing He, Kangli Zhu, Xiaojun Song, Ying Zhen, Enhui Shen, Dazhi Jin, Qiao Bian, Danyang Li, Yun Luo, Liang Tao, Zhenrui Pan |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Bacterial Toxins Medicine (miscellaneous) Clostridium difficile toxin B Biology Polymorphism Single Nucleotide Genome Article General Biochemistry Genetics and Molecular Biology Evolution Molecular Enterotoxins Mice 03 medical and health sciences Bacterial Proteins Phylogenetics Animals Humans Clinical microbiology lcsh:QH301-705.5 Peptide sequence Gene Phylogeny 030304 developmental biology Genetics 0303 health sciences Microbial toxins Whole Genome Sequencing Clostridioides difficile 030306 microbiology Infectious-disease diagnostics Clostridium difficile Subtyping Mice Inbred C57BL lcsh:Biology (General) Clostridium Infections General Agricultural and Biological Sciences Clostridioides Multilocus Sequence Typing |
| Zdroj: | Communications Biology, Vol 3, Iss 1, Pp 1-8 (2020) Communications Biology |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-020-1078-y |
| Popis: | Clostridioides difficile toxins (TcdA and TcdB) are major exotoxins responsible for C. difficile infection (CDI) associated diseases. The previously reported TcdB variants showed distinct biological features, immunoactivities, and potential pathogenicity in disease progression. Here, we performed global comparisons of amino acid sequences of both TcdA and TcdB from 3,269 C. difficile genomes and clustered them according to the evolutionary relatedness. We found that TcdB was much diverse and could be divided into eight subtypes, of which four were first described. Further analysis indicates that the tcdB gene undergoes accelerated evolution to maximize diversity. By tracing TcdB subtypes back to their original isolates, we found that the distribution of TcdB subtypes was not completely aligned with the phylogeny of C. difficile. These findings suggest that the tcdB genes not only frequently mutate, but also continuously transfer and exchange among C. difficile strains. Shen et al. compare the amino acid sequences of bacterial toxins TcdA and TcdB from 3,269 Clostridioides difficile genomes to identify four new TcdB subtypes. They find that TcdB was more diverse in amino acid sequence than TcdA. This study suggests that the tcdB genes not only frequently mutate, but they also continuously transfer and exchange among C. difficile strains. |
| Databáze: | OpenAIRE |
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