Induction of immediate early gene mRNAs and proteins by hCG in interstitial cells of rat testis
| Autor: | J Kononen, R Schultz, M Pelto-Huikko |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty JUNB Endocrinology Diabetes and Metabolism In situ hybridization Steroid biosynthesis Biology Chorionic Gonadotropin Immediate early protein Interstitial cell Immediate-Early Proteins Rats Sprague-Dawley Endocrinology Genes jun Internal medicine Gene expression Image Processing Computer-Assisted medicine Animals Genes Immediate-Early In Situ Hybridization Regulation of gene expression Genes fos Leydig Cells Immunohistochemistry Rats Gene Expression Regulation Immediate early gene |
| Zdroj: | Journal of Endocrinology. 144:417-424 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.1440417 |
| Popis: | In the present study we have investigated the induction of immediate early gene (IEG) c-fos, c-jun, junB and junD mRNAs and proteins in interstitial cells of rat testis after hCG treatment in vivo using in situ hybridization and immunocytochemistry. The basal levels of all IEGs were barely detectable with our methods. A prominent induction of c-fos, c-jun and junB mRNAs and proteins in Leydig cells was seen after hCG treatment. The induction of these IEG mRNAs and proteins demonstrates that their expression is an early transcriptional response to the extracellular stimulus of hCG and that they are involved in transcriptional processes which may mediate the effects of LH on cellular adaptive responses or steroid biosynthesis. Journal of Endocrinology (1995) 144, 417–424 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|