Assessment of anesthesia on physiological stability and bold signal reliability during visual or acoustic stimulation in the cat
| Autor: | Stephen G. Lomber, Blake E. Butler, Alexandra T. Levine, Benson Li, Paisley Barnes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Stimulation Stability (probability) 03 medical and health sciences 0302 clinical medicine Neuroimaging Functional neuroimaging medicine Anesthesia Ketamine 030304 developmental biology 0303 health sciences business.industry General Neuroscience Alfaxalone fMRI Animal models Physiological stability 030104 developmental biology Isoflurane Anesthetic business Stimulus-evoked 030217 neurology & neurosurgery BOLD medicine.drug |
| Zdroj: | Brain and Mind Institute Researchers' Publications |
| DOI: | 10.1101/827949 |
| Popis: | BackgroundNeuroimaging methods including fMRI provide powerful tools to observe whole-brain functional networks. This is particularly powerful in animal models, allowing these networks to be probed using complementary methods. However, most animals must be anesthetized for neuroimaging, giving rise to complications resulting from anesthetic effects on the animal’s physiological and neurological functions. For example, an established protocol for feline neuroimaging involves co-administration of ketamine and isoflurane – the latter of which is known to suppress cortical function.New MethodHere, we compare this established protocol to alfaxalone, a single-agent anesthetic for functional neuroimaging. We first compare the two in a controlled environment to assess relative safety and to measure physiological stability over an extended time window. We then compare patterns of auditory and visually-evoked activity measured at 7T to assess mean signal strength and between-subjects signal variability.Results in Comparison with Existing MethodsWe show that alfaxalone results in more stable respiratory rates over the 120 minutes testing period, with evidence of smaller between measurements variability within this time window, when compared to ketamine plus isoflurane. Moreover, we demonstrate that both agents evoke similar mean BOLD signals across animals, but that alfaxalone elicits more consistent BOLD activity in response to sound stimuli across all ROIs observed.ConclusionsAlfaxalone is observed to be more physiologically stable, evoking a more consistent BOLD signal across animals than the co-administration of ketamine and isoflurane. Thus, an alfaxalone-based protocol may represent a better approach for neuroimaging in animal models requiring anesthesia. |
| Databáze: | OpenAIRE |
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