Reactive Oxygen Species Produced by a Photodynamic Effect Induced Calcium Signal in Neurons and Astrocytes
| Autor: | E. V. Berezhnaya, Andrey Y. Abramov, Anatoly B. Uzdensky, Maria Neginskaya |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Porphyrins Thapsigargin SERCA Neuroscience (miscellaneous) chemistry.chemical_element Biology Calcium medicine.disease_cause Rats Sprague-Dawley Lipid peroxidation 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Animals Calcium Signaling Calcium signaling Neurons Photosensitizing Agents Phospholipase C Lasers Endoplasmic reticulum Coculture Techniques Rats Cell biology Drug Combinations 030104 developmental biology Neurology chemistry Astrocytes Reactive Oxygen Species Oxidative stress |
| Zdroj: | Molecular Neurobiology. 55:96-102 |
| ISSN: | 1559-1182 0893-7648 |
| Popis: | Photodynamic therapy (PDT) leads to production of reactive oxygen species (ROS) and cell destruction due to oxidative stress. We used photodynamic effect of photosensitizer radachlorin to unravel the effect of photo-induced oxidative stress on the calcium signal and lipid peroxidation in primary culture of cortical neurons and astrocytes using live cell imaging. We have found that irradiation in presence of 200 nM of radachlorin induces calcium signal in primary neurons and astrocytes. Photo-induced neuronal calcium signal depends on internal calcium stores as it was still observed in calcium-free medium and could be blocked by depletion of endoplasmic reticulum (ER) stores with inhibitor of sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) thapsigargin. Both inhibitors of phospholipase C activity U73122 and water-soluble analogue of vitamin E Trolox suppressed calcium response activated by PDT. We have also observed that the photodynamic effect of radachlorin induces lipid peroxidation in neurons and astrocytes. This data demonstrate that lipid peroxidation induced by PDT in neurons and astrocytes leads to activation of phospholipase C that results in production of inositol 1,4,5-trisphosphate (IP3). |
| Databáze: | OpenAIRE |
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