18F-Labeling of Mannan for Inflammation Research with Positron Emission Tomography
| Autor: | Riikka Siitonen, Anne Roivainen, Juhani Knuuti, Heidi Liljenbäck, Jörgen Bergman, Rikard Holmdahl, Outi Sareila, Helena E. Virtanen, Jouni Tuisku, Xiang-Guo Li, Cecilia Hagert |
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| Rok vydání: | 2016 |
| Předmět: |
ta3126
Pathology medicine.medical_specialty 010405 organic chemistry Chemistry Organic Chemistry Inflammation Spleen 010402 general chemistry medicine.disease 01 natural sciences Biochemistry In vitro 0104 chemical sciences carbohydrates (lipids) Psoriatic arthritis medicine.anatomical_structure In vivo Psoriasis Drug Discovery medicine Bone marrow medicine.symptom ta116 Mannan |
| Zdroj: | ACS Medicinal Chemistry Letters. 7:826-830 |
| ISSN: | 1948-5875 |
| DOI: | 10.1021/acsmedchemlett.6b00160 |
| Popis: | Recently mannan from Saccharomyces cerevisiae has been shown to be able to induce psoriasis and psoriatic arthritis in mice, and the phenotypes resemble the corresponding human diseases. To investigate the pathological processes, we set out to label mannan with fluorine-18 ((18)F) and study the (18)F-labeled mannan in vitro and in vivo with positron emission tomography (PET). Accordingly, mannan has been transformed into (18)F-fluoromannan with (18)F-bicyclo[6.1.0]nonyne. In mouse aorta, the binding of [(18)F]fluoromannan to the atherosclerotic lesions was clearly visualized and was significantly higher compared to blocking assays (P0.001) or healthy mouse aorta (P0.001). In healthy rats the [(18)F]fluoromannan radioactivity accumulated largely in the macrophage-rich organs such as liver, spleen, and bone marrow and the excess excreted in urine. Furthermore, the corresponding (19)F-labeled mannan has been used to induce psoriasis and psoriatic arthritis in mice, which indicates that the biological function of mannan is preserved after the chemical modifications. |
| Databáze: | OpenAIRE |
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