Myosin: immunofluorescent localization in neuronal and glial cultures
Autor: | Wanda Yorke, Fred J. Roisen, Margit Inczedy-Marcsek, Linda Hsu |
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Rok vydání: | 1978 |
Předmět: |
Neurons
Cytoplasm Multidisciplinary biology medicine.diagnostic_test Actin remodeling Fluorescent Antibody Technique macromolecular substances Myosins Microfilament Immunofluorescence Cell biology Cell Line Cell culture Ganglia Spinal Myosin biology.protein medicine Antibody Neuroglia Actin Intracellular |
Zdroj: | Science (New York, N.Y.). 199(4336) |
ISSN: | 0036-8075 |
Popis: | The distribution of intracellular myosin was studied by the double antibody immunofluorescence method in primary organotypic neuronal cultures and two established neuronal and glial cell lines. An array of parallel filaments aligned with the major cellular axis and a three-dimensional subsurface network were shown to react with two different myosin antibodies. The presence of myosin-rich filaments in regions known to contain actin filaments suggests that these proteins interact to generate the motive force in nonmuscle contractile systems. |
Databáze: | OpenAIRE |
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