Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources
| Autor: | Christopher R. Bilder, Peter C. Iwen, Steven H. Hinrichs, Emily L. Mccutchen, Baha Abdalhamid, Scott A. Koepsell |
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| Rok vydání: | 2020 |
| Předmět: |
2019-20 coronavirus outbreak
Veterinary medicine Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Pooling SARS CoV-2 030204 cardiovascular system & hematology Biology Article Specimen Handling Betacoronavirus 03 medical and health sciences Special Article COVID-19 Testing 0302 clinical medicine Medical Laboratory Personnel Humans Viral rna 030212 general & internal medicine Group testing Cycle threshold Clinical Laboratory Techniques Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 COVID-19 General Medicine 030220 oncology & carcinogenesis RNA Viral Coronavirus Infections AcademicSubjects/MED00690 030215 immunology |
| Zdroj: | American Journal of Clinical Pathology medRxiv |
| Popis: | Objectives To establish the optimal parameters for group testing of pooled specimens for the detection of SARS-CoV-2. Methods The most efficient pool size was determined to be five specimens using a web-based application. From this analysis, 25 experimental pools were created using 50 µL from one SARS-CoV-2 positive nasopharyngeal specimen mixed with 4 negative patient specimens (50 µL each) for a total volume of 250 µL. Viral RNA was subsequently extracted from each pool and tested using the CDC SARS-CoV-2 RT-PCR assay. Positive pools were consequently split into individual specimens and tested by extraction and PCR. This method was also tested on an unselected group of 60 nasopharyngeal specimens grouped into 12 pools. Results All 25 pools were positive with cycle threshold (Ct) values within 0 and 5.03 Ct of the original individual specimens. The analysis of 60 specimens determined that 2 pools were positive followed by identification of 2 individual specimens among the 60 tested. This testing was accomplished while using 22 extractions/PCR tests, a savings of 38 reactions. Conclusions When the incidence rate of SARS-CoV-2 infection is 10% or less, group testing will result in the saving of reagents and personnel time with an overall increase in testing capability of at least 69%. |
| Databáze: | OpenAIRE |
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