Nitric Oxide-Releasing Alginates
| Autor: | Mark H. Schoenfisch, Mona Jasmine R. Ahonen, Huanyu Zhu, Dakota J. Suchyta |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Staphylococcus aureus
Polymers and Plastics Alginates Cell Survival Carboxylic acid Kinetics Antineoplastic Agents Bioengineering 02 engineering and technology Nitric Oxide 010402 general chemistry 01 natural sciences Article Nitric oxide Biomaterials chemistry.chemical_compound Biopolymers Materials Chemistry Humans Amines Alkyl Cell Proliferation Carbodiimide chemistry.chemical_classification Aqueous solution 021001 nanoscience & nanotechnology Small molecule Anti-Bacterial Agents 0104 chemical sciences Molecular Weight chemistry A549 Cells Pseudomonas aeruginosa Amine gas treating 0210 nano-technology Azo Compounds Nuclear chemistry |
| Zdroj: | Biomacromolecules. 19:1189-1197 |
| ISSN: | 1526-4602 1525-7797 |
| Popis: | Low and high molecular weight alginate biopolymers were chemically modified to store and release potentially therapeutic levels of nitric oxide (NO). Carbodiimide chemistry was first used to modify carboxylic acid functional groups with a series of small molecule alkyl amines. The resulting secondary amines were subsequently converted to N-diazeniumdiolate NO donors via reaction with NO gas under basic conditions. NO donor-modified alginates stored between 0.4–0.6 µmol NO·mg(−1). In aqueous solution, the NO-release kinetics were diverse (0.3–13 h half-lives), dependent on the precursor amine structure. The liberated NO showed bactericidal activity against Pseudomonas aeruginosa and Staphylococcus aureus with pathogen eradication efficiency dependent on both molecular weight and NO-release kinetics. The combination of lower molecular weight (~5 kDa) alginates with moderate NO-release durations (half-life of ~4 h) resulted in enhanced killing of both planktonic and biofilm-based bacteria. Toxicity against human respiratory epithelial (A549) cells proved negligible at NO-releasing alginate concentrations required to achieve a 5-log reduction in viability in the biofilm eradication assay. |
| Databáze: | OpenAIRE |
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