Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells
| Autor: | Pascale Goupil, Razik Benouaret, Eric Goujon |
|---|---|
| Přispěvatelé: | Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Financement FEDER, Projet Phytomarc |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Hypersensitive response
Cell death Programmed cell death Proteases Physiology Plant defence education Plant Science Cycloheximide Biology Genes Plant Grape marc chemistry.chemical_compound Aprotinin Downregulation and upregulation Leucine Pepstatins Tobacco Genetics Extracellular [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Vitis Cells Cultured Disease Resistance Plant Diseases Plant Proteins Plant Extracts Hydrogen-Ion Concentration Protease inhibitors Tobacco BY-2 Molecular biology Up-Regulation Elicitor chemistry Biochemistry [SDE]Environmental Sciences Hypersensitive reaction Pepstatin Peptide Hydrolases |
| Zdroj: | Plant Physiology and Biochemistry Plant Physiology and Biochemistry, Elsevier, 2014, 77, pp.84-89. ⟨10.1016/j.plaphy.2014.01.021⟩ |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2014.01.021⟩ |
| Popis: | Plant Physiol. Biochem. ISI Document Delivery No.: AE5EJ Times Cited: 0 Cited Reference Count: 34 Benouaret, R. Goujon, E. Goupil, P. FUI: Fonds Unique Interministeriel We thank Celine Sac for help with the tobacco cell cultures and laboratory assistance. This work was supported by grants from the FUI: Fonds Unique Interministeriel. Elsevier france-editions scientifiques medicales elsevier Paris; International audience; Grape marc extract (GME) showed elicitor activity on suspension-cultured cells of tobacco. The BY-2 cells reacted to GME (0.25% and 0.125%) with a long-sustained pH rise in their growth medium. Using EGTA or LaCl3, we showed that extracellular alkalinization depended on Ca2+ mobilization. The tobacco BY-2 cells challenged with GME promoted cell death and the upregulation of defence-related genes such as PR3, PAL and CCoAOMT. Cell death rate was quantified using an experimental calibrated Evans Blue assay. The GME-induced cell death was dose-dependent and occurred in 24 h. Longer exposure increased the extent of tobacco cell death. To investigate a potential hypersensitive reaction, we tested the effect of various inhibitors of protein synthesis (cycloheximide) and proteases (aprotinin, pepstatin and E-64) on GME-induced cell death. All these chemicals reduced GME-induced cell death rate in 30 min. Overall, our findings indicate that GME elicits early perception events, defence reactions and cell death requiring protein synthesis and proteases. |
| Databáze: | OpenAIRE |
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