Proteomics-Based Comparative Mapping of the Secretomes of Human Brown and White Adipocytes Reveals EPDR1 as a Novel Batokine
| Autor: | Bente Klarlund Pedersen, Andreas Kjaer, Carsten H. Nielsen, Bhargav S. Prabhakar, Birgitte Holst, Annika Sanfridson, Lone Peijs, Tao Ma, Atul S. Deshmukh, Rafael Bayarri-Olmos, Daniel J. Drucker, Zachary Gerhart-Hines, Therese Juhlin Larsen, Mai Charlotte Krogh Severinsen, Naja Z. Jespersen, Matthias Mann, Jacqueline L. Beaudry, Mads Tang-Christensen, Charlotte Helgstrand, Peter Garred, Andreas D. Brunner, Søren Nielsen, Gilbert G. Privé, Camilla Scheele |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Proteomics Physiology Adipocytes White Adipose tissue Adipokine Nerve Tissue Proteins White adipose tissue Biology Transfection Cohort Studies 03 medical and health sciences Mice Young Adult 0302 clinical medicine Adipose Tissue Brown Animals Humans Molecular Biology Aged Mice Knockout Secretory Pathway Goiter Cell Biology Secretomics Middle Aged Cell biology Neoplasm Proteins Mice Inbred C57BL 030104 developmental biology Secretory protein Adipocytes Brown Adipogenesis Gene Knockdown Techniques Female Thermogenesis 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Cell Metabolism |
| ISSN: | 1932-7420 |
| Popis: | Summary Adipokines secreted from white adipose tissue play a role in metabolic crosstalk and homeostasis, whereas the brown adipose secretome is less explored. We performed high-sensitivity mass-spectrometry-based proteomics on the cell media of human adipocytes derived from the supraclavicular brown adipose and from the subcutaneous white adipose depots of adult humans. We identified 471 potentially secreted proteins covering interesting categories such as hormones, growth factors, extracellular matrix proteins, and proteins of the complement system, which were differentially regulated between brown and white adipocytes. A total of 101 proteins were exclusively quantified in brown adipocytes, and among these was ependymin-related protein 1 (EPDR1). EPDR1 was detected in human plasma, and functional studies suggested a role for EPDR1 in thermogenic determination during adipogenesis. In conclusion, we report substantial differences between the secretomes of brown and white human adipocytes and identify novel candidate batokines that can be important regulators of human metabolism. |
| Databáze: | OpenAIRE |
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