Revealing the dynamics of gene expression during embryonic genome activation and first differentiation in the rabbit embryo with a dedicated array screening

Autor: Cédric Cabau, Jean-Paul Renard, Annie Chastellier, R. D. Léandri, Véronique Duranthon, Catherine Archilla, L. C. Bui, Zichuan Liu, Nathalie Peynot
Přispěvatelé: Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Unité Mathématique Informatique et Génome (MIG), Institut National de la Recherche Agronomique (INRA), Laboratoire de radiobiologie et d'étude du génome (LREG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA), The work was supported by a fellowship awarded to R. D. Léandri by Institut National de la Santé et de la Recherche Médicale and by a grant from the Agence de Biomedecine (Recherche et PEGh 2006)., École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Physiological Genomics
Physiological Genomics, American Physiological Society, 2009, 36 (2), pp.98-113. ⟨10.1152/physiolgenomics.90310.2008⟩
Physiological Genomics, 2009, 36 (2), pp.98-113. ⟨10.1152/physiolgenomics.90310.2008⟩
Physiological Genomics 2 (36), 98-113. (2009)
ISSN: 1094-8341
1531-2267
Popis: Chantier qualité GA; International audience; Early mammalian development is characterized by extensive changes in nuclear functions that result from epigenetic modifications of the newly formed embryonic genome. While the first embryonic cells are totipotent, this status spans only a few cell cycles. At the blastocyst stage, the embryo already contains differentiated trophectoderm cells and pluripotent inner cell mass cells. Concomitantly, the embryonic genome becomes progressively transcriptionally active. During this unique period of development, the gene expression pattern has been mainly characterized in the mouse, in which embryonic genome activation (EGA) spans a single cell cycle after abrupt epigenetic modifications. To further characterize this period, we chose to analyze it in the rabbit, in which, as in most mammals, EGA is more progressive and occurs closer to the first cell differentiation events. In this species, for which no transcriptomic arrays were available, we focused on genes expressed at EGA and first differentiation and established a 2,000-gene dedicated cDNA array. Screening this with pre-EGA, early post-EGA, and blastocyst embryos divided genes into seven clusters of expression according to their regulation during this period and revealed their dynamics of expression during EGA and first differentiation. Our results point to transient properties of embryo transcriptome at EGA, due not only to the transition between maternal and embryonic transcripts but also to the transient expression of a subset of embryonic genes whose functions remained largely uncharacterized. They also provide a first view of the functional consequences of the changes in gene expression program.
Databáze: OpenAIRE
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