Effects of sphingosine-1-phosphate on gene expression of two cell mouse embryos induced by C2-Ceramide
| Autor: | Lei Guo, Weisen Zeng, Xianghong Ou, Chen Luo, Xujing Geng, Song Quan, Hong Li, Lan Ma |
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| Rok vydání: | 2014 |
| Předmět: |
Ceramide
Sphingosine-1-phosphate lcsh:QH471-489 Cell Pre-implantation mouse embryos Two cell embryo chemistry.chemical_compound Cyclin-dependent kinase Gene expression Obstetrics and Gynaecology medicine lcsh:Reproduction Gene lcsh:R5-920 biology Obstetrics and Gynecology Embryo Molecular biology medicine.anatomical_structure Reproductive Medicine chemistry biology.protein CDKN1B lcsh:Medicine (General) |
| Zdroj: | Middle East Fertility Society Journal, Vol 19, Iss 2, Pp 107-114 (2014) |
| ISSN: | 1110-5690 |
| DOI: | 10.1016/j.mefs.2013.06.001 |
| Popis: | Objective As to understand the sphingosine-1-phosphate (S1P) on gene expression of the pre-implantation mouse embryo induced by C 2 -Ceramide (CED). Materials and methods Global gene-expression profiling of two cell mouse embryo was performed using Affymetrix Gene Chip® Mouse Genome 430 2.0 Array. Results Fifty-five genes were identified with significant expression changes that are functionally involved in the two cell embryo after treated by S1P and CED. Of these genes, 30 were up-regulated and 25 were down-regulated. Genes Atm, Cdkn1b and Fgrfr2 are identified to be involved in the protective role of S1P against the apoptotic signals induced by CED. Conclusions This study provides a map of genes in the pre-implantation two cell mouse embryo. Further investigation based on these data will provide a better understanding of the effects of S1P on the pre-implantation embryos in other mammalian species, especially human. |
| Databáze: | OpenAIRE |
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