A physical map of the Mycoplasma agalactiae strain PG2 genome
| Autor: | Sebastiana Tola, Stefano Rocca, Guido Leori, G. Idini, Angela Maria Rocchigiani, Daniela Manunta |
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| Rok vydání: | 2001 |
| Předmět: |
Gel electrophoresis
Genetics XhoI General Veterinary ved/biology Mycoplasma agalactiae Restriction Mapping ved/biology.organism_classification_rank.species Chromosome Mapping General Medicine Biology Microbiology Molecular biology Genome Electrophoresis Gel Pulsed-Field SmaI Blotting Southern Restriction enzyme Mycoplasma Pulsed-field gel electrophoresis biology.protein Deoxyribonucleases Type II Site-Specific Genome Bacterial Southern blot |
| Zdroj: | Veterinary Microbiology. 80:121-130 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/s0378-1135(00)00389-8 |
| Popis: | We have constructed a physical map of the Mycoplasma agalactiae strain PG2 chromosome analyzing it by pulsed field gel electrophoresis in a contour-clamped homogeneous electric-field system. We mapped 33 cleavage sites generated with SmaI, XhoI, SalI, EclXI and BsiWI restriction endonucleases using double digestions, one- and two-dimensional pulsed electrophoresis, cross-hybridization and linking clones. We have also mapped the loci of some genes by Southern hybridization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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