Rapid Diagnosis of EnterohemorrhagicEscherichia coliO157:H7 Directly from Fecal Specimens Using Immunofluorescence Stain
| Autor: | Choong H. Park, W L Morrison, D L Hixon, C B Cook |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Serotype
Time Factors food.ingredient Fluorescent Antibody Technique Biology Immunofluorescence medicine.disease_cause Stain Microbiology Feces fluids and secretions food Antigen Escherichia coli medicine Humans Agar Child Direct fluorescent antibody Escherichia coli Infections Bacteriological Techniques medicine.diagnostic_test Infant General Medicine Enteritis Latex fixation test Child Preschool Gastrointestinal Hemorrhage |
| Zdroj: | American Journal of Clinical Pathology. 101:91-94 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/101.1.91 |
| Popis: | Serotype O157:H7 is most frequently encountered among verotoxin-producing Escherichia coli. Most laboratories use MacConkey-sorbitol agar as a screening medium. Presumptive identification of sorbitol-negative colonies is then accomplished by latex agglutination or biochemical tests with serologic confirmation, which requires 18-36 hours for completion. This study attempted to detect E coli O157:H7 directly from stool specimens by direct immunofluorescence (DIF) antibody staining to provide quicker turnaround (< 2 hours). A total of 336 abnormal fecal samples (bloody, watery, semi-liquid, or mucoid) were examined by this method. Results were compared with those of culture. Direct immunofluorescence antibody staining of the direct fecal smear detected all isolates of E coli O157 that were recovered by culture, including nonmotile strains, strains possessing the H7 flagellar antigen, and one strain with a flagellar antigen other than H7. Optimum results were achieved when specimens were pretreated with 5% bleach and centrifugation. No false-negative results were obtained with bleach-pretreated stool samples. |
| Databáze: | OpenAIRE |
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